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首页> 外文期刊>American journal of applied sciences >Ganoderma Species Associated with Basal Stem Rot Disease of Oil Palm
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Ganoderma Species Associated with Basal Stem Rot Disease of Oil Palm

机译：与油棕基茎腐烂病相关的灵芝物种

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Problem statement: Basal Stem Rot disease (BSR) is one of the most serious diseases that have been causing major losses in the oil palm industry in Southeast Asia, especially in Malaysia and Indonesia. Several species of Ganoderma have been reported pathogenic to oil palm, however, the diversity and differentiation of the Ganoderma species were not widely studied and the identity of these species are still unclear which may lead to inaccurate and inefficient decision-making in disease management. Approach: In this study, several isolates of Ganoderma were collected in Sarawak, Malaysia and the Multiplex Polymerase Chain Reaction was carried out to differentiate the isolates into species level. This was followed by morphological studies of basidiocarp of the Ganoderma isolates cultivated via artificial cultivation whereby parameters, such as basidiocarp and spore size, color and physical morphology were recorded. Results: Multiplex PCR could be used to differentiate the Ganoderma isolates, however, optimization had to be done to obtain convincing results. Morphology studies of the Ganoderma isolates showed that spore length could be used to distinguish among the Ganoderma species. Conclusion: Three Ganoderma species viz., G. boninense, G. zonatum and G. miniatocinctum are associated with the basal stem rot disease in Sarawak. Further studies on Ganoderma morphological traits is suggested so that immediate identification method can be developed to give guidance in deciding the most suitable way for control measures without any delay, leading to reduced palm deaths and disease control cost, thus, reducing losses in the oil palm industry.
机译：问题陈述：基底干腐病（BSR）是最严重的疾病之一，已引起东南亚尤其是马来西亚和印度尼西亚的油棕产业重大损失。已经报道了几种灵芝物种对油棕有致病性，但是，灵芝物种的多样性和分化尚未得到广泛研究，这些物种的身份仍然不清楚，这可能导致疾病管理决策不准确和效率低下。方法：在这项研究中，在马来西亚沙捞越州收集了几株灵芝分离株，并进行了多重聚合酶链反应以将这些分离株区分到物种水平。随后进行了通过人工栽培培养的灵芝分离株的基底皮的形态学研究，从而记录了诸如基底皮和孢子大小，颜色和物理形态等参数。结果：多重PCR可以用于区分灵芝分离物，但是，必须进行优化以获得令人信服的结果。灵芝分离物的形态学研究表明，孢子长度可用于区分灵芝物种。结论：砂拉越的三种灵芝物种，即邦尼氏菌，松果灵芝和小灵芝，与基底茎腐烂病有关。建议对灵芝的形态特征进行进一步研究，以便开发立即鉴定方法，为确定最合适的控制方法提供指导，而不会造成任何拖延，从而减少手掌死亡和疾病控制成本，从而减少油棕的损失。行业。

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