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首页> 外文期刊>American Journal of Potato Research >Isolation of Bacteria from the Rhizosphere and Rhizoplane of Potato (Solanum tuberosum) Grown in Two Distinct Soils Using Semi Selective Media and Characterization of Their Biological Properties
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Isolation of Bacteria from the Rhizosphere and Rhizoplane of Potato (Solanum tuberosum) Grown in Two Distinct Soils Using Semi Selective Media and Characterization of Their Biological Properties

机译：利用半选择培养基分离两种不同土壤中种植的马铃薯（马铃薯）的根际和根际平面中的细菌及其生物学特性

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The objective of this study was to isolate and characterize genera of bacteria that had been identified as being the most common residents in the rhizosphere of potato using cpn60 pyrosequencing analysis. Using various semi-selective media targeted to specific genera of interest, 200 isolates of bacteria were collected from rhizosphere soil and the rhizoplane of potatoes grown in soils obtained from a potato farm in Prince Edward Island and Ontario. The procedures employed were successful in selecting out representative bacteria suggested by pyrosequencing to be common in the potato rhizoplane. Results of 16S rRNA sequencing showed that 44 % of the isolates represented new species. All isolates were tested for biological and biochemical activities including phosphate solubilization, phytohormone metabolism, nitrogen fixation, antibiosis, exoenzyme production, and production of acyl-homoserine lactones. Massilia spp. and Chryseobacterium spp. showed the strongest exoenzyme activities. A greater proportion of Agrobacterium tumefaciens rhizosphere strains produced acyl-homoserine lactones compared to rhizoplane strains. Pseudomonas spp. and Lysobacter capsici had the greatest antagonistic activity on laboratory media towards six potato pathogens, and also significantly decreased disease in plants grown in pathogen-infested soil. Four isolates significantly increased growth of potato nodal explants in tissue culture. By using preliminary results derived from next generation sequencing technology and a targeted cultural technique, we were able to gain a better understanding of the biological activities of the most abundant bacterial species in the rhizosphere and rhizoplane of a cultivated crop.
机译：这项研究的目的是使用cpn60焦磷酸测序分析来分离和鉴定被鉴定为马铃薯根际最常见细菌的属。使用针对特定感兴趣属的各种半选择培养基，从根际土壤和从爱德华王子岛和安大略省的马铃薯农场获得的土壤中生长的马铃薯的根际平面中收集了200种细菌分离株。所采用的方法成功地选择出了焦磷酸测序所建议的代表性细菌，这些细菌在马铃薯根际平面中很常见。 16S rRNA测序的结果表明，有44％的分离物代表新物种。测试了所有分离物的生物学和生化活性，包括磷酸盐增溶，植物激素代谢，固氮，抗菌，外切酶生产和酰基高丝氨酸内酯的生产。 Massilia spp。和Chryseobacterium spp。表现出最强的外酶活性。与根际平面菌株相比，更大比例的根癌农杆菌根际菌株产生酰基-高丝氨酸内酯。假单胞菌辣椒杆菌和辣椒杆菌在实验室培养基上对六种马铃薯病原体具有最大的拮抗活性，并且还大大减少了在病原体侵染的土壤中生长的植物的病害。四个分离株显着增加了马铃薯节点外植体在组织培养中的生长。通过使用从下一代测序技术和有针对性的培养技术获得的初步结果，我们能够更好地了解栽培作物的根际和根际平面中最丰富的细菌物种的生物活性。

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