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首页> 外文期刊>Analytical and Bioanalytical Chemistry >UHPLC-ESI-MS/MS method for direct analysis of drugs of abuse in oral fluid for DUID assessment
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UHPLC-ESI-MS/MS method for direct analysis of drugs of abuse in oral fluid for DUID assessment

机译：UHPLC-ESI-MS / MS方法直接分析口服液中的滥用药物以进行DUID评估

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摘要

An ultra-high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry method for the direct analysis in oral fluid (OF) of several abused drugs and metabolites in a single chromatographic run was set up and validated. Amphetamine, methamphetamine, morphine, O-6-monoacetylmorphine, cocaine, codeine, methylenedioxymethamphetamine (MDMA), methylenedioxyethylamphetamine, methylenedioxyamphetamine, methadone, benzoylecgonine (BEG), Δ9-tetrahydrocannabinol (THC), ketamine, and cocaethylene were determined in a single chromatographic run with no sample pretreatment, after addition of the respective deuterated internal standards. The method was designed to perform a confirmation analysis on the residual OF samples after the preliminary on-site screening test, and it was applied on preservative buffers from different devices (Mavand Rapidstat, Concateno DDS, and Greiner Bio-One) or on neat OF samples. The method was suitable to be applied to the small amounts of sample available for the confirmatory analysis after the preliminary on-site screening or on undiluted OF samples. Limits of detection varied from 5 (morphine) to 0.2 ng/mL (methamphetamine, MDMA, BEG, and cocaethylene). The method was linear for all the substances involved, giving quadratic correlation coefficients of >0.99 in all the different preservative buffers checked. In addition, repeatability and accuracy were satisfactory for the majority of the substances, except for a few cases. The developed method was subsequently applied to 466 residual samples from on-site screening performed by police officers. Of these samples, 74 showed the presence of cocaine and metabolites; THC was detected in 49 samples. Two samples showed codeine and morphine while MDMA was detected in 11 samples and ketamine in four samples.
机译：建立并验证了一种超高效液相色谱-电喷雾电离-串联质谱法，可在一次色谱分析中直接分析几种滥用药物和代谢产物在口腔液中的含量。苯丙胺，甲基苯丙胺，吗啡，O-6-单乙酰吗啡，可卡因，可待因，亚甲基二氧甲基苯丙胺（MDMA），亚甲基二氧乙基苯丙胺，亚甲基二氧苯丙胺，美沙酮，苯甲酰基芽子碱（BEG），Δ9-四氢大麻酚（THC）和可卡因单酚添加相应的氘代内标后，无需进行样品预处理。该方法旨在在初步现场筛选测试后对残留的OF样品进行确认分析，并将其应用于不同设备（Mavand Rapidstat，Concateno DDS和Greiner Bio-One）或纯OF上的防腐缓冲液样品。该方法适用于在初步现场筛选后或未稀释的OF样品后用于确认分析的少量样品。检测限从5（吗啡）到0.2 ng / mL（甲基苯丙胺，MDMA，BEG和可可乙烯）不等。该方法对于所有涉及的物质都是线性的，在所检查的所有不同防腐剂缓冲液中的二次相关系数均> 0.99。此外，除少数情况外，大多数物质的重复性和准确性令人满意。随后，将开发的方法应用于警官现场筛查的466种残留样品。在这些样品中，有74个显示可卡因和代谢物的存在。在49个样品中检测到THC。两个样品显示可待因和吗啡，而在11个样品中检测到MDMA，在四个样品中检测到氯胺酮。

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