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首页> 外文期刊>Applied Microbiology and Biotechnology >Isolation and partial characterization of Bacillus subtilis ME488 for suppression of soilborne pathogens of cucumber and pepper
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Isolation and partial characterization of Bacillus subtilis ME488 for suppression of soilborne pathogens of cucumber and pepper

机译：抑制黄瓜和辣椒土传病原菌的枯草芽孢杆菌ME488的分离和部分鉴定

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摘要

Environmentally friendly control measures are needed for suppression of soilborne pathogens of vegetable crops in the Republic of Korea. In vitro challenge assays were used to screen approximately 500 bacterial isolates from 20 Korean greenhouse soils for inhibition of diverse plant pathogens. One isolate, Bacillus subtilis ME488, suppressed the growth of 39 of 42 plant pathogens tested. Isolate ME488 also suppressed the disease caused by Fusarium oxysporum f. sp. cucumerinum on cucumber and Phytophthora capsici on pepper in pot assays. Polymerase chain reaction was used to screen isolate ME488 for genes involved in biosynthesis of 11 antibiotics produced by various isolates of B. subtilis. Amplicons of the expected sizes were detected for bacD and bacAB, ituC and ituD, and mrsA and mrsM involved in the biosynthesis of bacilysin, iturin, and mersacidin, respectively. The identity of these genes was confirmed by DNA sequence analysis of the amplicons. Bacilysin and iturin were detected in culture filtrates from isolate ME488 by gas chromatography coupled with mass spectroscopy and by thin layer chromatography, respectively. Detection of mersacidin in ME488 culture filtrates was not attempted. Experiments reported here indicate that B. subtilis ME488 has potential for biological control of pathogens of cucumber and pepper possibly due to the production of antibiotics.
机译：大韩民国需要采取环境友好的控制措施来抑制蔬菜作物的土壤传播病原体。体外激发试验用于从20种韩国温室土壤中筛选出约500种细菌分离株，以抑制多种植物病原体。一种分离株，枯草芽孢杆菌ME488，抑制了测试的42种植物病原体中39种的生长。分离物ME488还抑制了尖孢镰刀菌f引起的疾病。 sp。在盆栽试验中，黄瓜在黄瓜上，辣椒在辣椒上。聚合酶链反应用于筛选分离物ME488中涉及枯草芽孢杆菌各种分离物产生的11种抗生素生物合成的基因。检测了预期大小的bacD和bacAB，ituC和ituD的扩增子，以及分别与杆菌肽，iturin和mersacidin的生物合成有关的mrsA和mrsM。这些基因的身份通过扩增子的DNA序列分析得到证实。通过气相色谱-质谱联用和薄层色谱法分别检测到分离物ME488的培养滤液中的杆菌素和尿素。未尝试检测ME488培养滤液中的过氧化胃酸。此处报道的实验表明，枯草芽孢杆菌ME488可能具有生物防治黄瓜和辣椒病原体的潜力，这可能是由于产生了抗生素所致。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2008年第1期|115-123|共9页
  • 作者

    Soohee Chung; Hyesuk Kong; Jeffrey S. Buyer; Dilip K. Lakshman; John Lydon; Sang-Dal Kim; Daniel P. Roberts;

  • 作者单位

    Department of Applied Microbiology Yeungnam University Gyeongsan 712-749 Gyeongbuk Republic of Korea;

    Laboratory of Methods Development CBER FDA Rockville MD 20852 USA;

    Sustainable Agricultural Systems Laboratory USDA—Agricultural Research Service Bldg. 001 Room 140 Beltsville Agricultural Research Service 10300 Baltimore Avenue Beltsville MD 20705-2350 USA;

    Florist and Nursery Plants Unit USDA—Agricultural Research Service Beltsville MD 20705 USA;

    Sustainable Agricultural Systems Laboratory USDA—Agricultural Research Service Bldg. 001 Room 140 Beltsville Agricultural Research Service 10300 Baltimore Avenue Beltsville MD 20705-2350 USA;

    Department of Applied Microbiology Yeungnam University Gyeongsan 712-749 Gyeongbuk Republic of Korea;

    Sustainable Agricultural Systems Laboratory USDA—Agricultural Research Service Bldg. 001 Room 140 Beltsville Agricultural Research Service 10300 Baltimore Avenue Beltsville MD 20705-2350 USA;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Bacillus subtilis; Bacilysin; Biological control; Fusarium oxysporum f. sp. cucumerinum; Iturin; Mersacidin; Methyl bromide replacement; Phytophthora capsici;

    机译：枯草芽孢杆菌;溶菌素;生物防治;尖孢镰刀菌f。 sp。黄瓜;Iturin;Meracidin;溴甲烷替代;辣椒疫霉;

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