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首页> 外文期刊>Applied Surface Science >Fischer carbene mediated covalent grafting of a peptide nucleic acid on gold surfaces and IR optical detection of DNA hybridization with a transition metalcarbonyl label
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Fischer carbene mediated covalent grafting of a peptide nucleic acid on gold surfaces and IR optical detection of DNA hybridization with a transition metalcarbonyl label

机译：费歇尔卡宾介导的金表面肽核酸的共价接枝和红外金属过渡金属羰基标记的光学杂交检测

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摘要

Amine-reactive surfaces comprising N-hydroxysuccinimide ester groups as well as much more unusual Fischer alkoxymetallocarbene groups were generated on gold-coated surfaces via self-assembled mono layers of carboxy- and azido-terminated thiolates, respectively. These functions were further used to immobilize homothymine peptide nucleic acid (PNA) decamer in a covalent fashion involving the primary amine located at its N-terminus. These stepwise processes were monitored by polarization modulation reflection - absorption infrared spectroscopy (PM-RAIRS) that gave useful information on the molecular composition of the organic layers. PNA grafting and hybridization with complementary DNA strand were successfully transduced by quartz crystal microbalance (QCM) measurements. Unfortunately, attempts to transduce the hybridization optically by IR in a label-free fashion were inconclusive. Therefore we undertook to introduce an IR reporter group, namely a transition metalcarbonyl (TMC) entity at the 5' terminus of complementary DNA. Evidence for the formation of PNA-DNA heteroduplex was brought by the presence of v(C equivalent to O) bands in the 2000 cm(-1) region of the IR spectrum of the gold surface owing to the metalcarbonyl label. (C) 2016 Elsevier B.V. All rights reserved.
机译：分别通过羧基和叠氮基封端的硫醇盐的自组装单层，在镀金表面上生成了包含N-羟基琥珀酰亚胺酯基以及更不寻常的Fischer烷氧基金属碳烯基的胺反应性表面。这些功能还用于以共价方式固定高胸腺嘧啶肽核酸（PNA）十聚体，涉及位于其N端的伯胺。通过偏振调制反射-吸收红外光谱（PM-RAIRS）监控这些逐步过程，该过程给出了有关有机层分子组成的有用信息。通过石英晶体微量天平（QCM）测量成功地转导了PNA嫁接和与互补DNA链的杂交。不幸的是，通过IR以无标记方式光学转导杂交的尝试尚无定论。因此，我们承诺在互补DNA的5'末端引入一个IR报告基团，即过渡金属羰基（TMC）实体。由于金属羰基标记的存在，金表面IR光谱的2000 cm（-1）区域中存在v（C等于O）带，从而提供了形成PNA-DNA异源双链体的证据。 （C）2016 Elsevier B.V.保留所有权利。

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