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Study on the binding equilibrium between surfactant FC95 and DNA by resonance light-scattering technique

机译:共振光散射技术研究表面活性剂FC95与DNA的结合平衡

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摘要

The binding equilibrium of potassium perfluorooctanesulfonate (FC95) to DNA was studied by UV spectra and resonance light scattering technique in the present paper. It was found that the interaction resulted in strongly enhanced resonance light scattering signals characterized by the RLS peak at 370 nm. The results indicated that the conformation of the DNA has changed during the interaction because the micro environment of DNA changed. The binding constant K_f and the number of binding n were calculated according to the equilibrium equation, which was established based on the data of the resonance light scattering measurements at 370 nm. It was found that the binding K_f and the number of binding n were 6.02×10~3 and 2.0, respectively.
机译:通过紫外光谱和共振光散射技术研究了全氟辛烷磺酸钾(FC95)与DNA的结合平衡。发现该相互作用导致共振光散射信号大大增强,其特征在于在370nm处的RLS峰。结果表明,在相互作用过程中,DNA的构象发生了变化,因为DNA的微观环境发生了变化。根据平衡方程计算结合常数K_f和结合数n,该平衡方程基于在370nm下的共振光散射测量的数据建立。发现结合K_f和结合数n分别为6.02×10〜3和2.0。

著录项

  • 来源
    《Applied Surface Science》 |2011年第10期|p.4547-4551|共5页
  • 作者单位

    Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University, Wuhan 430062, China;

    Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University, Wuhan 430062, China;

    Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University, Wuhan 430062, China;

    Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University, Wuhan 430062, China;

    Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University, Wuhan 430062, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Fluorinated surfactant; DNA; Resonance light scattering; Conformation;

    机译:氟化表面活性剂;脱氧核糖核酸;共振光散射;构象;

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