A label-free method for studying DNA sequence recognition of mitoxantrone based on resonance light-scattering technique

ChenZ.; ZhangG.; ChenX.; ChenJ.; LiuJ.

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A label-free method for studying DNA sequence recognition of mitoxantrone based on resonance light-scattering technique

机译：基于共振光散射技术研究米托蒽醌DNA序列识别的无标记方法

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A label-free method for studying DNA sequence recognition of mitoxantrone (MIT) by resonance light-scattering (RLS) technique has been developed in this contribution. Through the RLS spectra, the selective no-covalent interactions between MIT and double-stranded DNA, single-stranded DNA (ssDNA), oligonucleotides were systematically studied. The results of the experiments displayed that MIT had an obvious preference to ssDNA with the K (K RLS), 15.16 mmol mg -1 and the number of binding sites (N), 8.68 × 10 -4 mmol mg 1. Besides, it was found that MIT had a preference to sequences that were rich in guanine and cytosine bases with K RLS, 17.29 l mmol -1 and N, 1.19 × 10 -2 l mmol -1. The recognition mechanisms were well discussed and by fluorescence method and atom force microscopy, the RLS technique was confirmed to be a reliable method in this study. Compared with other methods, what the RLS strategy displayed was the direct interaction between anticancer drugs and DNA in vitro without the influence of a foreign substance. Thus, it can be a simple, fast and label-free strategy for DNA sequence recognition studies of DNA-targeted anticancer drugs.

机译：为此，开发了一种无标记方法，用于通过共振光散射（RLS）技术研究米托蒽醌（MIT）的DNA序列识别。通过RLS光谱，系统地研究了MIT与双链DNA，单链DNA（ssDNA）和寡核苷酸之间的选择性无共价相互作用。实验结果表明，MIT对ssDNA具有明显的偏爱，其K（K RLS）为15.16 mmol mg -1，结合位点数（N）为8.68×10 -4 mmol mg 1。发现MIT优先选择富含鸟嘌呤和胞嘧啶碱基且K RLS为17.29 l mmol -1和N为1.19×10 -2 l mmol -1的序列。对识别机理进行了很好的讨论，并通过荧光方法和原子力显微镜，RLS技术被认为是一种可靠的方法。与其他方法相比，RLS策略显示的是体外抗癌药物与DNA之间的直接相互作用而不受外来物质的影响。因此，对于靶向DNA的抗癌药物的DNA序列识别研究而言，这可能是一种简单，快速且无标签的策略。

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《The Journal of Antibiotics: An International Journal》 |2012年第10期|共6页
作者
ChenZ.; ZhangG.; ChenX.; ChenJ.; LiuJ.;
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正文语种 eng
中图分类药学;
关键词
anticancer drugs; mitoxantrone; oligonucleotides; recognition; resonance light scattering; selectivity;

机译：抗癌药米托蒽醌寡核苷酸识别共振光散射选择性;

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1. A label-free method for studying DNA sequence recognition of mitoxantrone based on resonance light-scattering technique [J] . ChenZ., ZhangG., ChenX., The Journal of Antibiotics: An International Journal . 2012,第10期

机译：基于共振光散射技术研究米托蒽醌DNA序列识别的无标记方法
2. Study on the binding equilibrium between surfactant FC95 and DNA by resonance light-scattering technique [J] . Ling Li, Quan Pan, Ying Xi Wang, Applied Surface Science . 2011,第10期

机译：共振光散射技术研究表面活性剂FC95与DNA的结合平衡
3. A graphene oxide-based sensing platform for the label-free assay of DNA sequence and exonuclease activity via long range resonance energy transfer [J] . Jiang Y., Tian J., Chen S., Journal of Fluorescence . 2013,第4期

机译：基于氧化石墨烯的传感平台，可通过远程共振能量转移进行DNA序列和核酸外切酶活性的无标记测定
4. Comparative Study of a DNA Sequence Storage Technique: An Improved Coding Method for Information Storage of DNA from Characters to Numbers [C] . Crystal Cob, Soo-Yeon Ji International Conference on Computational Science and Computational Intelligence . 2016

机译：DNA序列存储技术的比较研究：从字符到数字的DNA信息存储的改进编码方法
5. Improving the Production of Highly Custom Single-stranded DNA Sequences for Applications in DNA Nanotechnology through the Development of Novel Bacteriophage-based DNA-production Methods [D] . Nafisi, Parsa M. 2018

机译：通过开发新的基于噬菌体的DNA生产方法，提高高度定制的单链DNA序列的生产，以用于DNA纳米技术
6. In vitro recognition of DNA base pairs by histones in histone-DNA complexes and reconstituted core particles: an ultraviolet resonance Raman study. [O] . A Laigle, L Chinsky, P Y Turpin, 1982

机译：组蛋白-DNA复合物中组蛋白和重组核心颗粒在体外对DNA碱基对的识别：紫外线共振拉曼研究。
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8. Neural network: Multiple sensor based method for recognition of gene coding segments in human DNA sequence data. [R] . Uberbacher, E. C., Mann, R. C., Hand, R. C., 1991

机译：神经网络：基于多传感器的方法识别人类DNa序列数据中的基因编码区段。

A label-free method for studying DNA sequence recognition of mitoxantrone based on resonance light-scattering technique

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