• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Surface Science >High resolution SIMS imaging of cations in mammalian cell mitosis, and in Drosophila polytene chromosomes
【24h】

High resolution SIMS imaging of cations in mammalian cell mitosis, and in Drosophila polytene chromosomes

机译：高分辨率SIMS成像的哺乳动物细胞有丝分裂和果蝇多烯染色体中的阳离子。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The University of Chicago high resolution scanning ion microprobe (UC-SIM) was used to image, by Secondary Ion Mass Spectrometry (SIMS), the distribution of Ca2+ and Mg2+ in the chromosomes of Indian muntjac (IM) deer mitotic fibroblasts. This is part of a systematic study of the cation composition of mammalian cells and chromosomes throughout the cell cycle, after having shown that Ca2+ and Mg2+ appear to, be important for chromosome condensation and structure at metaphase. We focus here on a detailed description of the metaphase-anaphase transition at narrow time intervals beyond the G2/M border, made possible by controlled cell synchronization procedures. High-density distributions of chromosome spreads showed progressive stages of mitosis, identified by their morphology, within the same UC-SIM field of view. Subtle differences in cation contents between successive mitotic stages could thus be quantified in identical experimental conditions. Preliminary results indicate maximal chromosomal concentrations of Ca2+ and Mg2+ at metaphase, and a progressive decrease of the same with advancing stages of anaphase. Ca2+ and Mg2+ distributions were also imaged in the polytene chromosomes of Drosophila melanogaster, whose DNA distribution had been previously studied by BrdU labeling. These cations may play a common role in mitosis from lower eukaryotes to mammals. (c) 2006 Elsevier B.V. All rights reserved.
机译：芝加哥大学的高分辨率扫描离子微探针（UC-SIM）通过二次离子质谱（SIMS）成像，显示了印度Muntjac（IM）鹿有丝分裂成纤维细胞染色体中Ca2 +和Mg2 +的分布。这是对整个细胞周期内哺乳动物细胞和染色体阳离子组成的系统研究的一部分，此前已证明Ca2 +和Mg2 +似乎对染色体的凝结和中期结构很重要。在这里，我们将重点放在G2 / M边界之外的狭窄时间间隔内的中期-后期过渡的详细描述，这可以通过受控的小区同步过程来实现。在相同的UC-SIM视野中，染色体分布的高密度分布显示出有丝分裂的进行性阶段，通过其形态识别。因此，可以在相同的实验条件下量化连续有丝分裂阶段之间阳离子含量的细微差异。初步结果表明，中期Ca2 +和Mg2 +的最大染色体浓度，并随着后期的发展而逐渐降低。 Ca2 +和Mg2 +的分布在果蝇的多聚体染色体中也有图像，其DNA分布先前已通过BrdU标记进行了研究。这些阳离子可能在从低等真核生物到哺乳动物的有丝分裂中起共同作用。 （c）2006 Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号