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Initial bioadhesion on surfaces in the oral cavity investigated by scanning force microscopy

机译:扫描力显微镜研究口腔表面的初始生物粘附

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Scanning force microscopy (SFM) was used to measure the adhesion forces between BSA, a saliva protein, and two dental surfaces, natural enamel and a filling material (Dyract AP(TM)). Measurements were taken in phosphate buffered aqueous solutions (PBS). Forces were resolved down to the piconewton regime. The dependency of the adhesion force on the interaction time, pH-value and substrate surface was monitored. In a further step, surface samples were fixed on an enamel brace and carried for a defined time in the oral cavity. The formed biofilm, called pellicle, shows a different morphology on the different substrates. This can be explained by the above-mentioned substrate dependence of the adhesion force. (c) 2005 Elsevier B.V. All rights reserved.
机译:扫描力显微镜(SFM)用于测量BSA,唾液蛋白和两个牙齿表面,天然牙釉质和填充材料(Dyract AP TM)之间的粘附力。在磷酸盐缓冲水溶液(PBS)中进行测量。部队被归结为皮科顿政权。监测粘附力对相互作用时间,pH值和底物表面的依赖性。在进一步的步骤中,将表面样品固定在牙釉质支架上,并在口腔中运送规定的时间。形成的被称为防护膜的生物膜在不同的基材上显示出不同的形态。这可以通过上述基材对粘附力的依赖性来解释。 (c)2005 Elsevier B.V.保留所有权利。

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