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首页> 外文期刊>Aquatic Sciences >Endospore-forming bacteria as new proxies to assess impact of eutrophication in Lake Geneva (Switzerland-France)
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Endospore-forming bacteria as new proxies to assess impact of eutrophication in Lake Geneva (Switzerland-France)

机译：形成内生孢子的细菌作为评估日内瓦湖富营养化影响的新代理（瑞士-法国）

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摘要

Measurements of chemical composition and biological parameters of sediment cores are used as proxies for changes in past environmental conditions and more recently the human impact on ecosystem health. In this study, endospore-forming bacteria are proposed as a new biological proxy for such paleoecological reconstructions. A sediment core providing a record for the past 90 years (~(137)Cs and magnetic susceptibility dating) was retrieved from the Rhone Delta of Lake Geneva. X-ray fluorescence was analyzed at a 0.2-cm resolution, while DNA extracts, elemental geochemistry and grain size were obtained at 4-cm intervals. The total number of bacteria and endospore-forming bacteria were quantified by qPCR using the 16S rRNA gene and the endosporulation-specific spo0A gene. Furthermore, a spo0A fragment was subjected to amplicon sequencing to define OTUs (operational taxo-nomic units) and the phylogenetic affiliation of the endospore formers. The results showed that despite the fact that the quantity of extracted DNA decreased with the age of the sediment, the abundance of endospore-forming bacteria remained constant. However, the diversity of this group of bacteria changed significantly, reflecting the eutrophication of the lake from 1960 to 1990. The shift in community composition was linked to the dominance of anaerobic clostridia-like endospore formers. This trend has reversed in the last 10 years of the record, suggesting a recovery after perturbation. This study shows that the abundance and diversity of endospore-forming bacteria can be used as proxies to reconstruct lake history. We hereby successfully introduce a new strategy for paleoecology that could also be applied to ocean sediments and long sediment cores.
机译：沉积物芯的化学成分和生物学参数的测量值被用作过去环境条件以及最近人类对生态系统健康影响的变化的代表。在这项研究中，提出了形成内生孢子的细菌作为这种古生态重建的一种新的生物替代物。从日内瓦湖的罗纳河三角洲取回了一个沉积芯，该芯提供了过去90年的记录（〜（137）Cs和磁化率测年）。 X射线荧光以0.2厘米的分辨率进行分析，而DNA提取物，元素地球化学和晶粒尺寸则以4厘米的间隔获得。使用16S rRNA基因和内生孢子特异性spo0A基因通过qPCR定量细菌和形成内生孢子的细菌总数。此外，对spo0A片段进行扩增子测序，以定义OTU（操作分类单位）和内生孢子形成物的系统发育关系。结果表明，尽管提取的DNA的数量随沉积物的年龄而减少，但形成内生孢子的细菌的丰度却保持不变。但是，这组细菌的多样性发生了显着变化，反映了1960年至1990年该湖的富营养化。群落组成的变化与厌氧梭状芽孢杆菌样内生孢子的形成有关。在记录的最后十年中，这种趋势发生了逆转，表明扰动后有所恢复。这项研究表明，内生孢子形成细菌的丰富性和多样性可以用作重建湖泊历史的代理。我们在此成功引入了一种新的古生态学策略，该策略也可以应用于海洋沉积物和长沉积物核心。

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  • 来源
    《Aquatic Sciences》 |2014年第1期|S103-S116|共14页
  • 作者

    Tina Wunderlin; Juan Pablo Corella; Thomas Junier; Matthieu Bueche; Jean-Luc Loizeau; Stephanie Girardclos; Pilar Junier;

  • 作者单位

    Laboratory of Microbiology, University of Neuchatel, Rue Emile Argand 11, 2000 Neuchatel, Switzerland;

    Department of Geology and Paleontology, Institute for Environmental Sciences (ISE), University of Geneva, 1205 Geneve, Switzerland,Museo Nacional de Ciencias Naturales (MNCN/CSIC), Serrano 115bis, 28006 Madrid, Spain;

    Laboratory of Microbiology, University of Neuchatel, Rue Emile Argand 11, 2000 Neuchatel, Switzerland;

    Laboratory of Microbiology, University of Neuchatel, Rue Emile Argand 11, 2000 Neuchatel, Switzerland;

    Institute F. A. Forel, Earth and Environmental Sciences Section, Faculty of Sciences, University of Geneva, Geneva, Switzerland;

    Department of Geology and Paleontology, Institute for Environmental Sciences (ISE), University of Geneva, 1205 Geneve, Switzerland;

    Laboratory of Microbiology, University of Neuchatel, Rue Emile Argand 11, 2000 Neuchatel, Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Endospore-forming bacteria; Paleoecological proxy; Sediment record; Lake Geneva; Human impact; Eutrophication;

    机译：形成孢子的细菌;古生态代理泥沙记录;日内瓦湖;对人类的影响;富营养化;

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