• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of Toxicology >Lutein improves antioxidant defense in vivo and protects against DNA damage and chromosome instability induced by cisplatin
【24h】

Lutein improves antioxidant defense in vivo and protects against DNA damage and chromosome instability induced by cisplatin

机译：叶黄素改善体内的抗氧化防御能力，并防止顺铂引起的DNA损伤和染色体不稳定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lutein (LT) is the second most prevalent carotenoid in human serum, and it is abundantly present in dark, leafy green vegetables. The objectives of this study were to evaluate the genotoxicity and mutagenicity of LT, and its protective effects in vivo against DNA damage and chromosome instability induced by cisplatin (cDDP). For this purpose, we used the comet assay and micronucleus (MN) test, and we evaluated the antioxidant effects of LT by determination of enzymatic (catalase—CAT) and non-enzymatic (reduced glutathione—GSH) activity. Mice were divided into six groups: cDDP, mineral oil (OM), LT groups and LT + cDDP groups. To perform the MN test on peripheral blood (PB) cells, blood samples were collected before the first treatment (T0), and 36 h (T1) and 14 days (T2) after the first treatment. To perform the comet assay, blood samples were collected 4 h after the first and the last treatment. Oxidative capacity was analyzed in total blood that was collected 24 h after the last treatment, when bone marrow (BM) sample was also collected for the MN test. No genotoxic or mutagenic effects of LT were observed for the doses evaluated. We did find that this carotenoid was able to reduce the formation of crosslinks and chromosome instability induced by cDDP. No differences were observed in CAT levels, and LT treatment increased GSH levels compared with a negative control group, reinforcing the role of this carotenoid as an antioxidant.
机译：叶黄素（LT）是人类血清中第二大最普遍的类胡萝卜素，它大量存在于深色多叶绿色蔬菜中。这项研究的目的是评估LT的遗传毒性和致突变性，以及其在体内对顺铂（cDDP）诱导的DNA损伤和染色体不稳定性的保护作用。为此，我们使用了彗星试验和微核（MN）测试，并通过确定酶促（过氧化氢酶-CAT）和非酶促（还原型谷胱甘肽-GSH）活性来评估LT的抗氧化作用。将小鼠分为六组：cDDP，矿物油（OM），LT组和LT + cDDP组。为了对外周血（PB）细胞进行MN测试，在第一次治疗之前（T0）以及第一次治疗之后36 h（T1）和14天（T2）收集血液样本。为了进行彗星试验​​，在第一次和最后一次治疗后4小时收集血样。最后一次治疗后24小时收集的全血中的氧化能力进行了分析，当时还收集了骨髓（BM）样本用于MN测试。对于所评估的剂量，未观察到LT的遗传毒性或诱变作用。我们确实发现该类胡萝卜素能够减少cDDP诱导的交联形成和染色体不稳定性。与阴性对照组相比，CAT水平没有差异，LT治疗可增加GSH水平，从而增强了这种类胡萝卜素作为抗氧化剂的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号