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首页> 外文期刊>Archives of Toxicology >Hormetic acute response and chronic effect of ethanol on adenine nucleotide hydrolysis in rat platelets
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Hormetic acute response and chronic effect of ethanol on adenine nucleotide hydrolysis in rat platelets

机译：乙醇对大鼠血小板腺嘌呤核苷酸水解的急性急性反应和慢性作用

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摘要

The objective of this study was to verify the acute and chronic effects of ethanol on platelet NTPDase and 5′-nucleotidase activities. These enzymes modulate platelet function by regulating adenine nucleotide bioavailability and adenosine production. In the acute treatment, doses of 0.8, 2.0, 4.0, 6.0 and 8.0 g/kg ethanol were administered via orogastric tube, and induced a biphasic or hormetic effect on ATP, ADP and AMP platelet hydrolysis. Ethanol at a dose of 0.8 and 2.0 g/kg increased NTPDase activity (44 and 35%, P < 0.0001) with ATP as substrate, whereas when ADP was used there was only a tendency for NTPDase activity to increase. ATP and ADP hydrolysis decreased by 31–77% (P < 0.0001) in 4.0, 6.0 and 8.0 g/kg of ethanol compared to the control. AMP hydrolysis showed a tendency to increase at ethanol doses of 0.8 and 2.0 g/kg, but was inhibited by 45–100% (P < 0.0001) at the higher doses. Chronic treatment consisted of the oral administration of 20% ethanol solution during 31 weeks as the only source of liquid and inhibited NTPDase activity (15 and 20%, P < 0.05) with ATP and ADP as substrate, respectively. However, AMP hydrolysis by 5′-nucleotidase increased by 40% (P < 0.05). Thus, we speculate that the effects of ethanol on NTPDase and 5′-nucleotidase activities could be related with the platelets alterations commonly observed in alcohol users.
机译：这项研究的目的是验证乙醇对血小板NTPDase和5'-核苷酸酶活性的急性和慢性作用。这些酶通过调节腺嘌呤核苷酸的生物利用度和腺苷产生来调节血小板功能。在急性治疗中，经口胃管给予0.8、2.0、4.0、6.0和8.0 g / kg乙醇，对ATP，ADP和AMP血小板水解产生双相或抑制作用。以ATP为底物，乙醇浓度为0.8和2.0 g / kg时，NTPDase活性增加（44和35％，P <0.0001），而当使用ADP时，NTPDase活性只会增加。与对照组相比，在4.0、6.0和8.0 g / kg乙醇中，ATP和ADP水解降低31-77％（P <0.0001）。 AMP水解在0.8和2.0 g / kg的乙醇剂量下显示增加的趋势，但是在较高剂量下被抑制45-100％（P <0.0001）。慢性治疗包括在31周内口服20％乙醇溶液作为唯一液体来源，并以ATP和ADP为底物分别抑制NTPDase活性（分别为15％和20％，P <0.05）。但是，5'-核苷酸酶的AMP水解增加了40％（P <0.05）。因此，我们推测乙醇对NTPDase和5'-核苷酸酶活性的影响可能与酒精使用者中常见的血小板改变有关。

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