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SSR Marker-Based DNA Fingerprinting and Cultivar Identification of Olives (Olea europaea)

机译:基于SSR标记的橄榄DNA指纹图谱和品种鉴定(油橄榄)

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摘要

Four well-known commercial olive cultivars (Domat, Edremit, Gemlik, and Memecik) and six local cultivars (Ziraat, Isrange, Tuz, Patos, Yag, and Marantelli) from northeastern Turkey were analyzed for genetic diversity and relationships using seven SSR primers (DCA-4, DCA-09, DCA-11, DCA-16, DCA-17, GAPU-89, UDO-14). The number of markers ranged from 3 (DCA-04 and DCA-17) to 6 (DCA-11, DCA-16, GAPU-89), with an average of 4.57 alleles per primer. UPGMA cluster analysis based on a simple matching similarity matrix grouped cultivars into two main clusters. Three pairs of cultivars (Ziraat and Gemlik, Isrange and Tuz, and Patos and Yag) were thought to be different cultivars although they produced identical SSR profiles. The results indicate the efficiency of SSR markers for evaluation of genetic diversity in olives and identification of misnamed individuals of the same genotype.
机译:使用七个SSR引物分析了来自土耳其东北部的四个著名的商业橄榄品种(D​​omat,Edremit,Gemlik和Memecik)和六个本地品种(Ziraat,Isrange,Tuz,Patos,Yag和Marantelli)的遗传多样性和亲缘关系( DCA-4,DCA-09,DCA-11,DCA-16,DCA-17,GAPU-89,UDO-14)。标记数范围从3个(DCA-04和DCA-17)到6个(DCA-11,DCA-16,GAPU-89),每个引物平均有4.57个等位基因。基于简单匹配相似度矩阵的UPGMA聚类分析将品种分为两个主要聚类。尽管三对品种(Ziraat和Gemlik,Isrange和Tuz以及Patos和Yag)具有相同的SSR谱,但被认为是不同的品种。结果表明,SSR标记可用于评估橄榄的遗传多样性并鉴定相同基因型的误名个体。

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