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首页> 外文期刊>Biochemical Genetics >Molecular Cloning and Characterization of a Novel Human Hydroxysteroid Dehydrogenase-Like 2 (HSDL2) cDNA from Fetal Brain
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Molecular Cloning and Characterization of a Novel Human Hydroxysteroid Dehydrogenase-Like 2 (HSDL2) cDNA from Fetal Brain

机译：胎儿脑中新型人类羟类固醇脱氢酶样2（HSDL2）cDNA的分子克隆和表征

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摘要

Hydroxysteroid dehydrogenases (HSDs) are responsible for the biosynthesis of steroid hormones and play a crucial role in mammalian physiology and development. By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a novel human hydroxysteroid dehydrogenase-like cDNA (HSDL2). This cDNA is 3211 bp in length, encoding a 418–amino-acid polypeptide, which contains a typical motif for NAD(P)+-binding (TGxxxGxG), an SDR active site motif (S-Y-K) and a sterol carrier protein domain. HSDL2 shows high similarity with the homologues in the mouse and fruit fly. The HSDL2 gene is mapped to chromosome 9q32 and contains 11 exons. RT-PCR analysis shows that the HSDL2 gene is widely expressed in human tissues and the expression levels in liver, kidney, prostate, testis, and ovary are relatively high.
机译：羟基类固醇脱氢酶（HSD）负责类固醇激素的生物合成，并在哺乳动物的生理和发育中起关键作用。通过人类胎儿脑cDNA文库的大规模测序分析，我们分离了一种新型的人类羟类固醇脱氢酶样cDNA（HSDL2）。该cDNA全长3211 bp，编码418个氨基酸的多肽，其中包含NAD（P）+ -结合的典型基序（TGxxxGxG），SDR活性位点基序（SYK）和固醇载体蛋白质结构域。 HSDL2与小鼠和果蝇的同源物显示出高度相似性。 HSDL2基因被定位到9q32染色体，并包含11个外显子。 RT-PCR分析表明，HSDL2基因在人体组织中广泛表达，在肝，肾，前列腺，睾丸和卵巢中的表达水平较高。

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