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首页> 外文期刊>Biochemistry >The Crystal Structure of Escherichia coli Group 4 Capsule Protein GfcC Reveals a Domain Organization Resembling That of Wza
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The Crystal Structure of Escherichia coli Group 4 Capsule Protein GfcC Reveals a Domain Organization Resembling That of Wza

机译：大肠杆菌第4组胶囊蛋白GfcC的晶体结构揭示了类似于Wza的域组织

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摘要

We report the 1.9 Å resolution crystal structure of enteropathogenicnEscherichia coli GfcC, a periplasmic protein encoded by the gfc operon, which is essentialnfor assembly of group 4 polysaccharide capsule (O-antigen capsule). Presumed genenorthologs of gfcC are present in capsule-encoding regions of at least 29 genera of Gram-nnegative bacteria. GfcC, a member of the DUF1017 family, is comprised of tandem β-graspn(ubiquitin-like) domains (D2 and D3) and a carboxyl-terminal amphipathic helix, a domainnarrangement reminiscent of that of Wza that forms an exit pore for group 1 capsule export.nUnlike themembrane-spanning C-terminal helix fromWza, the GfcC C-terminal helix packsnagainst D3. Previously unobserved in a β-grasp domain structure is a 48-residue helicalnhairpin insert inD2 that binds to D3, constraining its position and sequestering the carboxyl-nterminal amphipathic helix. A centrally located and invariant Arg115 not only is essential for proper localization but also forms one ofntwo mostly conserved pockets. Finally, we draw analogies between a GfcC protein fused to an outer membrane β-barrel pore innsome species and fusion proteins necessary for secreting biofilm-forming exopolysaccharides.
机译：我们报告肠致病性大肠杆菌GfcC（一种由gfc操纵子编码的周质蛋白）的1.9Å分辨率晶体结构，这对于组装第4组多糖胶囊（O抗原胶囊）至关重要。 gfcC的一般基因同源物存在于革兰氏阴性菌至少29属的胶囊编码区中。 GfcC是DUF1017家族的成员，由串联β-graspn（泛素样）结构域（D2和D3）和羧基末端两亲性螺旋结构组成，该结构域结构让人联想到Wza的结构域形成了第1组的出口孔与Wza的跨膜C末端螺旋不同，GfcC C末端螺旋紧贴D3。先前在β-抓图结构域中未观察到的是在D2中有一个48个残基的螺旋状发夹插入物，该插入物与D3结合，限制了其位置并螯合了羧基-n末端两亲性螺旋。位于中心且不变的Arg115不仅对于正确定位至关重要，而且还形成了两个最保守的口袋之一。最后，我们在融合到外膜β-桶状孔内体物种的GfcC蛋白与分泌形成生物膜的胞外多糖所必需的融合蛋白之间进行类比。

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  • 来源
    《Biochemistry》 |2011年第24期|p.5465-5476|共12页
  • 作者

    Karthik Sathiyamoorthy Erez Mills Titus M. Franzmann Ilan Rosenshine and Mark A. Saper;

  • 作者单位

    †Program in Biophysics, University of Michigan, Ann Arbor, Michigan 48109-1055, United States‡Department of Microbiology and Molecular Genetics, The Institute for Medical Research, Hebrew University of Jerusalem,91120 Jerusalem, Israel§Department of Molecular, Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, United States) Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, United States;

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  • 正文语种 eng
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  • 关键词

    Escherichia coli,Protein;

    机译：大肠杆菌;蛋白质;

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