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首页> 外文期刊>Bioenergy research >Identification of Fusarium oxysporum Genes Associated with Lignocellulose Bioconversion Competency
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Identification of Fusarium oxysporum Genes Associated with Lignocellulose Bioconversion Competency

机译：与木质纤维素生物转化能力相关的尖孢镰刀菌基因的鉴定

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摘要

Fusarium oxysporum can convert straw to ethanol via consolidated bioprocessing (CBP)—a two-stage process that firstly involves aerobic saccharification and thereafter an oxygen-limiting fermentation phase. The efficacy of CBP is dependent upon the fungal strain used. Using suppression subtractive hybridisation (SSH), a total of 210 transcripts were identified as being overexpressed in a high as compared to low efficacy CBP strain in the aerobic and oxygen-limiting growth stages on a straw/bran mix. These transcripts encode proteins assigned to various categories, including carbohydrate metabolism, energy, protein and sugar transport and detoxification. Real-time RT-PCR analysis of 12 transcripts, including an endoglucanase III (EGIII), a novel ricin toxin A1 chain-like protein (RTA1), and two unknown transcripts (JX308289 and JX308290) validated the SSH findings. Post-transcriptional silencing of EGIII, RTA1 and an unknown transcript JX308289 in F. oxysporum strain 11C significantly reduced the capacity of the fungus to produce ethanol from a straw/bran mix. Thus these and other genes identified in this study are likely factors that determine the efficacy of CBP and such genes can be used as candidates for enhancing microbial ethanol production from straw and other lignocellulosic substrates.
机译：尖孢镰刀菌可以通过整合生物处理（CBP）将秸秆转化为乙醇，这是一个分为两个阶段的过程，首先涉及有氧糖化，然后进入限氧发酵阶段。 CBP的功效取决于所使用的真菌菌株。使用抑制消减杂交（SSH），在秸秆/麸皮混合物的需氧和限氧生长阶段，与低效CBP菌株相比，共鉴定出210个转录本高表达。这些转录本编码分配给各种类别的蛋白质，包括碳水化合物代谢，能量，蛋白质和糖的运输和排毒。对12种转录本进行实时RT-PCR分析，包括内切葡聚糖酶III（EGIII），新型蓖麻毒素A1链状蛋白（RTA1）和两个未知转录本（JX308289和JX308290）证实了SSH的发现。尖孢镰刀菌菌株11C中EGIII，RTA1和未知转录本JX308289的转录后沉默显着降低了真菌从秸秆/麸皮混合物中生产乙醇的能力。因此，本研究中鉴定出的这些基因和其他基因可能是决定CBP功效的因素，这些基因可用作增强秸秆和其他木质纤维素底物的微生物乙醇生产的候选者。

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  • 来源
    《Bioenergy research》 |2014年第1期|110-119|共10页
  • 作者

    Shahin S. Ali; Mojibur Khan; Ewen Mullins; Fiona M. Doohan;

  • 作者单位

    1.Molecular Plant-Microbe Interactions Laboratory School of Biology and Environmental Science University College Dublin Dublin 4 Ireland 2.USDA/ARS Sustainable Perennial Crops Lab BARC-West Bldg 001 Beltsville MD 20705 USA;

    1.Molecular Plant-Microbe Interactions Laboratory School of Biology and Environmental Science University College Dublin Dublin 4 Ireland 4.Institute of Advanced Study in Science and Technology Guwahati-35 India;

    3.Department of Crop Science Teagasc Crop research Centre Oak Park Carlow Ireland;

    1.Molecular Plant-Microbe Interactions Laboratory School of Biology and Environmental Science University College Dublin Dublin 4 Ireland;

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  • 正文语种 eng
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  • 关键词

    Ethanol Fungi Transcriptome Lignocellulose Straw RTA1-like protein;

    机译：乙醇真菌转录组木质纤维素秸秆RTA1样蛋白;

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