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首页> 外文期刊>Journal of general plant pathology >Identification of genes with changes in transcription levels caused by mutations in conidiation regulator genes REN1 and FoSTUA in Fusarium oxysporum.
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Identification of genes with changes in transcription levels caused by mutations in conidiation regulator genes REN1 and FoSTUA in Fusarium oxysporum.

机译：鉴定由尖孢镰刀菌的分生调节基因REN1和FoSTUA突变引起的转录水平变化的基因。

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摘要

Fusarium oxysporum produces three kinds of asexual spores, macroconidia, microconidia, and chlamydospores. We previously identified two genes, REN1 and FoSTUA, which encode different types of transcription regulators essential for conidiation in F. oxysporum. We also performed expressed sequence tag analysis during vegetative growth and conidiation and identified 496 genes specifically detected in the conidiation cDNA library. Here, we selected genes with expression levels affected by a mutation in REN1 or FoSTUA in F. oxysporum. The transcription levels of 496 conidiation library-specific genes were compared between the wild-type strain and the Delta REN1 mutant or the Delta FoSTUA mutant by cDNA dot-blot differential hybridization and real-time quantitative PCR analyses. We found 23 and 12 genes, which had transcription levels more than tenfold lower and higher, respectively, in the Delta REN1 mutant than in the wild type. This result suggested that the 23 were positively and 12 genes negatively regulated by Ren1. We also found three genes positively regulated by FoStuA: the transcription levels of these genes were more than tenfold lower in the Delta FoSTUA mutant. The Ren1-regulated genes included genes that were also upregulated specifically under conidiation conditions in the wild-type strain. These genes provide candidates for further studies directed at understanding fungal conidiogenesis and its molecular regulation.
机译：尖孢镰刀菌产生三种无性孢子，大分生孢子，微分生孢子和衣原体孢子。我们以前鉴定了两个基因REN1和FoSTUA，它们编码对尖孢镰刀菌进行分生必不可少的不同类型的转录调节因子。我们还在营养生长和分生孢子形成过程中进行了表达序列标签分析，鉴定了在分生孢子cDNA文库中特异性检测到的496个基因。在这里，我们选择了基因表达水平受青枯菌中REN1或FoSTUA突变影响的基因。通过cDNA点印迹差异杂交和实时定量PCR分析，比较了野生型菌株和Delta REN1突变体或Delta FoSTUA突变体中496个分娩文库特异性基因的转录水平。我们发现了23个和12个基因，它们的Delta REN1突变体的转录水平分别比野生型低10倍和更高。该结果表明，Ren1正调控23个基因，负12个基因负调控。我们还发现了三个受FoStuA阳性调控的基因：这些基因的转录水平在Delta FoSTUA突变体中降低了十倍以上。 Ren1调控的基因包括野生型菌株在分生条件下也被上调的基因。这些基因为进一步研究真菌分生孢子发生及其分子调控提供了候选。

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