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首页> 外文期刊>Biology Bulletin >Ontogenetic and Phylogenetic Analysis of Myosin Light Chain Proteins from Skeletal Muscles of Loach Misgurnus fossilis
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Ontogenetic and Phylogenetic Analysis of Myosin Light Chain Proteins from Skeletal Muscles of Loach Misgurnus fossilis

机译：泥Mi化石骨骼肌中肌球蛋白轻链蛋白的本体遗传学和系统发育分析

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摘要

mRNAs of all three types of myosin light chain proteins are expressed in skeletal muscles of both larval and adult stages of loach Misgurnus fossilis (Cobitidae) and these proteins are encoded by different genes (mlc1, mlc2, and mlc3). No difference was revealed between transcripts from larval stage and adult fish for all three MLC proteins. Our approach (RT-PCR with fish-specific mlc1, mlc2, and mlc3 primers) failed to reveal the larval form of myosin light chain protein found previously by protein electrophoresis of loach fry muscle extract. Comparative analysis of the protein structure shows high homology of MLC1 and MLC3 proteins sharing a large EF-hand calcium-binding domain. Phylogenetic analysis of MLC1 from skeletal muscles of fish and other vertebrate species is concordant with the traditional phylogeny of-the group. Within the Teleostei, loach MLC1 had the highest homology with other Cyprinidae, and least with Salmonidae fishes.
机译：所有三种类型的肌球蛋白轻链蛋白的mRNA在泥Mi化石（Cobitidae）的幼虫和成年阶段的骨骼肌中表达，这些蛋白由不同的基因（mlc1，mlc2和mlc3）编码。对于所有三种MLC蛋白，幼虫阶段和成年鱼类的转录本之间均未发现差异。我们的方法（使用鱼特异性mlc1，mlc2和mlc3引物进行RT-PCR）未能揭示泥previously鱼苗肌肉提取物的蛋白质电泳以前发现的肌球蛋白轻链蛋白的幼体形式。蛋白质结构的比较分析显示，MLC1和MLC3蛋白质具有很高的同源性，它们具有较大的EF手钙结合结构域。对鱼类和其他脊椎动物物种骨骼肌中MLC1的系统发育分析与该组的传统系统发育相一致。在Teleostei中，泥achMLC1与其他鲤科的同源性最高，与鲑鱼的同源性最低。

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