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首页> 外文会议>ICoMST 2011;International conference of meat science and technology >Differences in myosin heavy chain mRNA expression levels among chicken muscles reflect differences in protein polymerization by transglutaminase
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Differences in myosin heavy chain mRNA expression levels among chicken muscles reflect differences in protein polymerization by transglutaminase

机译：鸡肌肉中肌球蛋白重链mRNA表达水平的差异反映了转谷氨酰胺酶在蛋白质聚合中的差异

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In this study we investigated the differences in microbial transglutaminase (MTG)-induced protein polymerization levels of the major muscle protein myosin heavy chain (MHC), in relation to their mRNA ratios, among different chicken muscles, and quantified their mRNA expression levels. SDS-PAGE demonstrated that MHC from these muscles differed in terms of its response to MTG. The expression levels of seven different MHC genes were investigated in four different muscles (pectoralis major, biceps femoris, gizzard and heart), using real-time-polymerase chain reaction analysis. The results indicated variations in expression of these genes, in terms of both specificity and quantity. The dominant genes in the pectoralis and biceps muscles were MyHC-6 and MyHC-1, respectively. Gizzard muscle expressed only MyHC-11, but this was expressed at high levels, and was also the most highly expressed gene among all the muscles. MyHC-7 was expressed only in cardiac muscle, though this muscle also expressed other MHC genes. The relationship between MHC mRNA expression and protein specificity suggests that the same proteins are responsible for different biological and biochemical reactions in different muscles. These results also indicate that variations in MHC mRNA expression parallel to the MHC protein specificity in different chicken muscles, and may account for the different MTG reactivities demonstrated by SDS-PAGE. We conclude that variations in gelation properties induced by transglutaminase between muscles reflect the different MHC protein levels, which in turn reflect the differences in MHC mRNA ratios among the different muscles.
机译：在这项研究中，我们调查了微生物转谷氨酰胺酶（MTG）诱导的主要肌肉蛋白肌球蛋白重链（MHC）的蛋白质聚合水平与它们在不同鸡肉中的mRNA比率之间的差异，并定量了它们的mRNA表达水平。 SDS-PAGE表明，来自这些肌肉的MHC对MTG的反应有所不同。使用实时聚合酶链反应分析，研究了四种不同肌肉（胸大肌，股二头肌，g和心脏）中七个不同MHC基因的表达水平。结果表明，就特异性和数量而言，这些基因的表达存在差异。胸大肌和二头肌的主要基因分别是MyHC-6和MyHC-1。 izz肌肉仅表达MyHC-11，但其表达水平很高，并且也是所有肌肉中表达最高的基因。 MyHC-7仅在心肌中表达，尽管该肌肉也表达其他MHC基因。 MHC mRNA表达与蛋白质特异性之间的关系表明，相同的蛋白质在不同的肌肉中负责不同的生物学和生化反应。这些结果还表明，MHC mRNA表达的变化与不同鸡肌肉中MHC蛋白的特异性平行，并且可能解释了SDS-PAGE所显示的MTG反应性不同。我们得出的结论是，转谷氨酰胺酶在肌肉之间引起的胶凝特性变化反映了不同的MHC蛋白水平，进而反映了不同肌肉之间MHC mRNA比率的差异。

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