A Simplified Method of Determination of cAMP in Plants Using Modified Enzyme Immunoassay

L. A. Lomovatskaya; A. S. Romanenko; N. V. Krivolapova

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A Simplified Method of Determination of cAMP in Plants Using Modified Enzyme Immunoassay

机译：修饰酶免疫法测定植物中cAMP的简化方法

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Amodification of enzyme immunoassay for quantification of cAMP and its derivatives in plants is suggested. It is based on the use of cAMP-specific primary rabbit antibodies and secondary goat antibodies con-jugated with peroxidase. The sensitivity of this method is 10 pM. This technique is highly specific, simple, and inexpensive.

机译：建议对酶免疫分析进行修饰以定量植物中的cAMP及其衍生物。它基于使用cAMP特异性一抗和过氧化酶偶联的山羊二抗。此方法的灵敏度为10 pM。该技术是高度特定，简单且廉价的。

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《Biology Bulletin》 |2005年第6期|p.567-570|共4页
作者
L. A. Lomovatskaya; A. S. Romanenko; N. V. Krivolapova;
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作者单位

Siberian Institute of Plant Physiology and Biochemistry, Siberian Division, Russian Academy of Sciences, ul. Lermontova 132, Irkutsk, 664033 Russia;

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正文语种 eng
中图分类生物科学;
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专利

1. Determination of cAMP in plant cells by a modified enzyme immunoassay method [J] . L. A. Lomovatskaya, A. S. Romanenko, N. V. Filinova, Plant Cell Reports . 2011,第1期

机译：改良酶免疫法测定植物细胞中的cAMP
2. Determination of cAMP in plant cells by a modified enzyme immunoassay method. [J] . Lomovatskaya L. A., Romanenko A. S., Filinova N. V., Plant Cell Reports . 2011,第1期

机译：改性酶免疫测定法测定植物细胞中的阵营。
3. Determination of blood sirolimus concentrations in liver and kidney transplant recipients using the Innofluor fluorescence polarization immunoassay: comparison with the microparticle enzyme immunoassay and high-performance liquid chromatography-ultraviolet method. [J] . Bouzas L, Hermida J, Tutor JC Upsala journal of medical sciences. . 2009,第1期

机译：使用Innofluor荧光偏振免疫测定法测定肝肾移植受者的血西罗莫司浓度：与微粒酶免疫测定法和高效液相色谱-紫外线法的比较。
4. Determination of deoxynivalenol in grain sorghum by chemiluminescence enzyme immunoassay [C] . Zhenzhen CHEN, Lixin ZHU, Renrong LIU, International Conference on Materials Engineering, Manufacturing Technology and Control . 2016

机译：化学发光酶免疫测定法测定谷物高粱中的脱氧苯酚
5. The determination of one-carbon metabolic intermediates in plants and the development of a protocol for the identification of pyridoxal-l-phosphate modified residues in proteins. [D] . Simon, Eric Steven. 2004

机译：植物中一碳代谢中间体的测定以及蛋白质中吡ido醛-1-磷酸修饰残基的鉴定方法的开发。
6. Determination of blood sirolimus concentrations in liver and kidney transplant recipients using the Innofluor® fluorescence polarization immunoassay: Comparison with the microparticle enzyme immunoassay and high-performance liquid chromatography-ultraviolet method [O] . Lorena Bouzas, Jesús Hermida, J Carlos Tutor 2009

机译：使用Innofluor®荧光偏振免疫测定法测定肝肾移植受者的西罗莫司浓度：与微粒酶免疫测定法和高效液相色谱-紫外线法的比较
7. Determination of blood sirolimus concentrations in liver and kidney transplant recipients using the Innofluor® fluorescence polarization immunoassay: Comparison with the microparticle enzyme immunoassay and high-performance liquid chromatography-ultraviolet method [O] . Bouzas, Lorena, Hermida, Jesús, Tutor, J Carlos 2009

机译：使用Innofluor®荧光偏振免疫测定法测定肝肾移植受者的西罗莫司浓度：与微粒酶免疫测定法和高效液相色谱-紫外线法的比较
8. Evaluation and Survey of Enzyme Immunoassay and Fluorometric Enzyme Immunoassay Tests for TSH and HCG. [R] . Armbruster, D., Harris, R., Scarbrough, R., 1989

机译：TsH和HCG酶免疫分析和荧光酶免疫分析试验的评价和调查。

A Simplified Method of Determination of cAMP in Plants Using Modified Enzyme Immunoassay

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