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首页> 外文期刊>Biology Bulletin >Biodiagnostics of Soil under a Low Level of Anthropogenic Impact (by the Example of the Urban Settlement of Stepnoe, Saratov Oblast)
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Biodiagnostics of Soil under a Low Level of Anthropogenic Impact (by the Example of the Urban Settlement of Stepnoe, Saratov Oblast)

机译：低水平的人为影响下土壤生物亚诊断（通过斯蒂尔诺伊的城市沉降，萨拉托夫州）

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Biodiagnostics of soil in a settlement with low technogenic impact was performed by the example of the urban settlement of Stepnoe. The number of microorganisms was evaluated in 30 soil samples by inoculation on solid nutrition media: on meat peptone agar for the calculation of the total heterotrophic microorganisms and on the corresponding selective media for hydrocarbon-oxidizing and iron-oxidizing microorganisms. We also analyzed the activity of several soil redox and hydrolytic enzymes (dehydrogenases, catalases, peroxidases, and invertases). The content of the mobile forms of copper, zinc, lead, cadmium, chromium, and nickel was determined to assess the anthropogenic impact on the soil, and the total soil contamination coefficient (Z(c)) was calculated. The excess of the MPC of mobile forms of Ni, Cu, and Pb was revealed in the area studied. In general, the total soil contamination coefficient did not exceed 16, indicating favorable environmental and geochemical conditions in the settlement. The analysis of the total number of heterotrophic microorganisms and the amounts of hydrocarbon-oxidizing and iron-oxidizing microorganisms showed deviations from the norm in some regions of the area surveyed, which pointed to anthropogenic transformation of the soil biocenoses. The maximum deviations were typical for the index of hydrocarbon-oxidizing microorganisms, which pointed to soil contamination by hydrocarbons. Our data on the evaluation of the activity of indicator soil enzymes did not exclude possible functional disturbances in the soil. It was found that the activity of soil dehydrogenases and invertases corresponded to low and very low levels of soil enrichment with these enzymes. The activity of soil catalases and peroxidases corresponded to the moderate and high levels of the enzymes, which indicated the absence of pathological changes in the soil. It has been shown that the studied soil microbiological and biochemical parameters are promising for soil health biodiagnostics in areas with low anthropogenic impact.
机译：通过斯蒂诺城市沉降的例子进行了低介绍影响的沉降中土壤的生物亚金目。通过在固体营养介质上接种在30种土样品中在30种土样品中评价微生物的数量：在肉蛋白胨琼脂上计算总异养微生物，并在相应的烃氧化和氧化铁微生物上的选择性培养基。我们还分析了几种土壤氧化还原和水解酶（脱氢酶，过缩酶，过氧化物酶和转化酶）的活性。测定铜，锌，铅，镉，铬，铬和镍的移动形式的含量评估对土壤的人为撞击，并计算整个土壤污染系数（Z（c））。在研究的地区揭示了MPC的MPC的MPC。通常，土壤污染系数的总污染系数不超过16，表明沉降中的有利环境和地球化学条件。对异养微生物的总数和烃氧化量的分析显示，氧化铁化微生物的偏差显示出对地区的一些地区的常态，这指出了土壤生物植物的人为转化。烃氧化微生物指数的最大偏差是典型的，其指向碳氢化合物的土壤污染。我们关于评价指标土壤酶活性的数据并未排除土壤中可能的功能性紊乱。发现土壤脱氢酶的活性和转化酶的活性对应于这些酶的低且极低的土壤富集。土壤过失和过氧化物酶的活性对应于中等和高水平的酶，这表明土壤中没有病理变化。已经表明，研究的土壤微生物和生化参数是对具有低人代抗冲击地区土壤健康生物诊断的有前途。

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