Methy-sens Comet assay and DNMTs transcriptional analysis as a combined approach in epigenotoxicology

Perotti Alessio; Rossi Valeria; Mutti Antonio; Buschini Annamaria

首页> 外文期刊>Biomarkers >Methy-sens Comet assay and DNMTs transcriptional analysis as a combined approach in epigenotoxicology

【24h】

Methy-sens Comet assay and DNMTs transcriptional analysis as a combined approach in epigenotoxicology

机译：Methy-sens Comet试验和DNMTs转录分析作为表观毒理学的组合方法

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Epigenotoxicology needs simple and fast tools to assess xenobiotic epigenetic load. This work proposes a comet assay modification designed to detect global methylation changes (Methysens Comet) through enzymatic digestion with two restriction enzymes (HpaII, MspI). In the methylation-sensitive protocol tested for repeatability on A549 cells, nickel chloride induced hypermethylation and decitabine-induced hypomethylation. A concomitant assessment of DNA methyltransferases (DNMTs) genes transcriptional levels has been performed, to implement a multifunctional approach to epigenotoxicology. Methy-sens Comet showed a general good repeatability and sensitivity to methylation changes while DNMTs transcriptional levels granted additional proof of xenobiotic-induced impairment of methylome maintenance.

机译：表观毒理学需要简单快捷的工具来评估异种表观遗传量。这项工作提出了一种彗星测定法修改方案，旨在通过两种限制性内切酶（HpaII，MspI）的酶消化来检测总体甲基化变化（Methysens彗星）。在甲基化敏感性方案中测试了A549细胞的可重复性，氯化镍诱导了甲基化过度，地西他滨诱导了甲基化不足。已经进行了DNA甲基转移酶（DNMT）基因转录水平的伴随评估，以实现表观毒理学的多功能方法。 Methy-sens彗星显示出良好的可重复性和对甲基化变化的敏感性，而DNMT的转录水平为异种生物诱导的甲基化维持受损提供了更多证据。

著录项

来源
《Biomarkers》 |2015年第8期|64-70|共7页
作者
Perotti Alessio; Rossi Valeria; Mutti Antonio; Buschini Annamaria;
展开▼
作者单位

Univ Parma, Dept Life Sci, I-43124 Parma, Italy|Univ Parma, Dept Clin & Expt Med, I-43124 Parma, Italy;

Univ Parma, Dept Life Sci, I-43124 Parma, Italy;

Univ Parma, Dept Clin & Expt Med, I-43124 Parma, Italy;

Univ Parma, Dept Life Sci, I-43124 Parma, Italy;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类
关键词
Comet assay; DNMT; epigenotoxicology; methylation;

机译：彗星试验;DNMT;表毒学;甲基化;

相似文献

外文文献
中文文献
专利

1. A Level Set Approach Combined with Support Vector Machine for Comet Detection from Comet Assay Images [J] . Sreelatha G., Sudarsan Manoj Kumar, Sathidevi P. S. Journal of Medical Imaging and Health Informatics . 2016,第4期

机译：一种水平集方法与支持向量机相结合的彗星试验图像检测
2. Validation of an automatic comet assay analysis system integrating the curve fitting of combined comet intensity profiles. [J] . Dehon G, Catoire L, Duez P, Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 2008,第2期

机译：验证了结合了彗星强度轮廓曲线拟合的自动彗星分析系统的有效性。
3. Combining the in vivo comet and micronucleus assays: a practical approach to genotoxicity testing and data interpretation [J] . Marie Z. Vasquez Mutagenesis . 2010,第2期

机译：结合体内彗星和微核试验：遗传毒性测试和数据解释的实用方法
4. Automatic detection of comets in Silver stained comet assay images for DNA damage analysis [C] . Sreelatha G., Rashmi P., Sathidevi P.S., IEEE International Conference on Signal Processing, Communications and Computing . 2014

机译：自动检测银染彗星测定图像中的彗星以进行DNA损伤分析
5. Mathematical modeling and analysis of genomic transcription assays. [D] . Gadgil, Chetan Jayant. 2001

机译：基因组转录分析的数学建模和分析。
6. Combining the in vivo comet and micronucleus assays: a practical approach to genotoxicity testing and data interpretation [O] . Marie Z. Vasquez -1

机译：结合体内彗星和微核试验：遗传毒性测试和数据解释的实用方法
7. Comet Methy-sens and DNMTs transcriptional analysis as a combined approach in epigenotoxicology [O] . Alessio Perotti, Valeria Rossi 2015

机译：彗星methy-sens和DNmTs转录分析作为表观活性毒理学的组合方法

Methy-sens Comet assay and DNMTs transcriptional analysis as a combined approach in epigenotoxicology

摘要

著录项

相似文献

相关主题

期刊订阅