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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enhanced production of carotenoids using a Thraustochytrid microalgal strain containing high levels of docosahexaenoic acid-rich oil
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Enhanced production of carotenoids using a Thraustochytrid microalgal strain containing high levels of docosahexaenoic acid-rich oil

机译：使用含有高含量二十二碳六烯酸的油的破囊壶菌微藻菌株增强类胡萝卜素的生产

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摘要

Results to date suggest that microalgal Thraustochytrids family strains can be used to produce high-functional omega-3 rich oil (similar to 30-70% of dry cell weight) and carotenoid-based antioxidant pigments simultaneously with value-added bioactive potential. In the present study, we describe the isolation and characterization of a new Thraustochytrid Schizochytrium sp. from the west coastal area of Korea. This newly isolated Thraustochytrid, identified as Schizochytrium sp. through 18S rRNA analysis and named SH104, simultaneously produces high levels of DHA and carotenoid-based antioxidant pigments. An improved Schizochytrium mutant, named SHG104, was obtained from the original host strain by gamma-irradiation-induced mutagenesis. Under combined temperature-shift cultivation conditions employing white-light LEDs (light-emitting diodes), Schizochytrium sp. SHG104 yielded 10.8 g L-1 of biomass comprising 45.8% total lipids (32.1% DHA) and 4.6 mg L-1 of astaxanthin. In addition to DHA, the main fatty acids produced by Schizochytrium sp. SHG104 were palmitic acid and a trace of other long-chain fatty acids. The carotenoid profile of SH104 and SHG104 was beta-carotene, astaxanthin, canthaxanthin, pheonicoxanthin and echinenone, which analyzed by HPLC and LC/APCI-MS. Furthermore, genomic analysis of Schizochytrium and Aurantiochytrium microalgae confirmed that the presence of carotenogenesis pathway enzymes and genes including geranylgeranyl diphosphate, phytoene synthase, lycopene cyclase, and cytochrome P450 hydroxylase that necessary for the production of antioxidants via a complete biosynthetic KEGG synthesis pathway. This newly isolated Schizochytrium microalga potentially have wide application as a source of antioxidants for astaxanthin-containing pigments, commercial omega-3 lipids and feed additives, such as nutritional supplements for aquaculture.
机译：迄今为止的结果表明，微藻类囊藻类家族菌株可用于生产高功能的富含omega-3的油（约占干细胞重量的30-70％）和类胡萝卜素类抗氧化剂颜料，并具有增值的生物活性潜力。在本研究中，我们描述了新的破囊壶菌Schizochytrium sp的分离和鉴定。来自韩国西部沿海地区。这种新近分离的破囊壶菌，鉴定为Schizochytrium sp。通过18S rRNA分析并命名为SH104，可同时生产高含量的DHA和类胡萝卜素抗氧化剂。通过γ射线诱导的诱变从原始宿主菌株中获得了改良的裂殖壶菌突变体SHG104。在采用白光LED（发光二极管）的组合温移栽培条件下，Schizochytrium sp.。 SHG104产生10.8 g L-1的生物质，其中包含45.8％的总脂质（32.1％DHA）和4.6 mg L-1的虾青素。除DHA外，由Schizochytrium sp。生产的主要脂肪酸。 SHG104是棕榈酸和其他痕量的长链脂肪酸。 SH104和SHG104的类胡萝卜素特征是β-胡萝卜素，虾青素，角黄素，苯并黄嘌呤和海胆烯酮，通过HPLC和LC / APCI-MS分析。此外，对裂殖壶菌和金鱼藻微藻进行的基因组分析证实，存在通过完整的生物合成KEGG合成途径生产抗氧化剂所必需的类胡萝卜素生成酶和基因，包括香叶基香叶基二磷酸，八氢番茄红素合酶，番茄红素环化酶和细胞色素P450羟化酶。这种新近分离的裂殖壶菌微藻潜在地广泛用作抗氧化剂的来源，用于含虾青素的颜料，商业的ω-3脂质和饲料添加剂，例如水产养殖的营养补品。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2018年第9期|1355-1370|共16页
  • 作者

    Park Hansung; Kwak Minsoo; Seo JeongWoo; Ju JeongHyun; Heo SunYeon; Park SeungMoon; Hong WonKyung;

  • 作者单位

    Chonbuk Natl Univ, Div Biotechnol, Coll Environm & Bioresource Sci, Iksan 570752, Jeonbuk, South Korea;

    Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Daejeon 305701, South Korea;

    KRIBB, Jeonbuk Branch Inst, Appl Microbial Res Ctr, Jeongeup 580185, Jeonbuk, South Korea;

    KRIBB, Jeonbuk Branch Inst, Appl Microbial Res Ctr, Jeongeup 580185, Jeonbuk, South Korea;

    KRIBB, Jeonbuk Branch Inst, Appl Microbial Res Ctr, Jeongeup 580185, Jeonbuk, South Korea;

    Chonbuk Natl Univ, Div Biotechnol, Coll Environm & Bioresource Sci, Iksan 570752, Jeonbuk, South Korea;

    Chonbuk Natl Univ, Div Biotechnol, Coll Environm & Bioresource Sci, Iksan 570752, Jeonbuk, South Korea;

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  • 正文语种 eng
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  • 关键词

    Thraustochytrid Schizochytrium; Antioxidant; Astaxanthin; DHA; Carotenogenesis;

    机译：破囊壶菌裂殖壶菌;抗氧化剂;虾青素;DHA;类胡萝卜素;

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