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首页> 外文期刊>Bioprocess and Biosystems Engineering >Hydrodynamic investigation of a novel shear-generating device for the measurement of anchorage-dependent cell adhesion intensity
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Hydrodynamic investigation of a novel shear-generating device for the measurement of anchorage-dependent cell adhesion intensity

机译：用于测量锚固依赖性细胞粘附强度的新型剪切产生装置的流体动力学研究

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摘要

Proliferation of anchorage-dependent cells occurs after adhesion to a suitable surface. Thus, quantitative information about the force of cells adhesion to microcarriers at early culture phases is vital for scaling up such system. In this work, a newly designed shear-generating device was proposed, based on a previously proposed contraction flow device designed for suspended cells. A design equation for the new device was also proposed to correlate the generated energy dissipation rate (EDR) with the cross-sectional area and flow rate. Microscopic-particle image velocimetry was measured to validate the simulation results, and good agreement was achieved. The newly designed device was then used to measure the adhesion force of MDCK and PK cells, and the results showed that the critical EDR was 3000 W/m(3) for MDCK and 5000 W/m(3) for PK cells. This quantitative information is of great value for better understanding shearing effects during the scaling up of anchorage-dependent cell cultures.
机译：粘附至合适表面后，发生锚固依赖性细胞的增殖。因此，关于在早期培养阶段细胞粘附于微载体的力的定量信息对于扩大此类系统至关重要。在这项工作中，基于先前提出的用于悬浮细胞的收缩流动装置，提出了一种新设计的剪切产生装置。还提出了新设备的设计方程，以将产生的能量耗散率（EDR）与横截面积和流速相关联。测量了显微图像测速仪以验证模拟结果，并取得了良好的一致性。然后，新设计的设备用于测量MDCK和PK细胞的粘附力，结果表明，对于MDCK，临界EDR为3000 W / m（3），对于PK细胞为5000 W / m（3）。该定量信息对于更好地了解依附性依赖性细胞培养物的放大过程中的剪切效应具有重要价值。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2018年第9期|1371-1382|共12页
  • 作者

    Wang Han; Xia Jianye; Zheng Zhiming; Zhuang Ying-Ping; Yi Xiaoping; Zhang Dahe; Wang Peng;

  • 作者单位

    Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, POB 1138,Comprehens Lab Bldg,350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;

    Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, POB 1138,Comprehens Lab Bldg,350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;

    Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, POB 1138,Comprehens Lab Bldg,350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Animal cell culture; Adhesion force; Energy dissipation rate; Microcarriers; Modeling;

    机译：动物细胞培养;黏附力;耗能率;微载体;建模;

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