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首页> 外文期刊>Bioprocess and Biosystems Engineering >Simultaneous decolorization and deproteinization of α,ω- dodecanedioic acid fermentation broth by integrated ultrafiltration and adsorption treatments
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Simultaneous decolorization and deproteinization of α,ω- dodecanedioic acid fermentation broth by integrated ultrafiltration and adsorption treatments

机译：综合超滤和吸附处理法同时对α，ω-十二碳二酸发酵液进行脱色和脱蛋白

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摘要

alpha,omega-Dicarboxylic acids (DC) are versatile chemical intermediates with different chain length. For biosynthesis of DC, to obtain the highly pure product via crystallization, it is required to remove pigments and proteins in fermentation broth. However, a trade-off between decolorization/deproteinization ratio and DC recovery during the purification process was found, which impeded DC production by fermentation. When ultrafiltration (UF) was applied to treat alpha,omega-dodecanedioic acid (DC12) broth, 93.4% of DC12 recovery, 80.5% of decolorization ratio and 61.7% of deproteinization ratio were achieved by a PES 3 membrane. However, the membrane technology could not effectively retain the pigments or proteins with low molecular weight when a high DC12 permeation was required. Meanwhile, the selected activated charcoal or macroporous resins were not good adsorbents for the present system. Furthermore, an integrated process for decolorization and deproteinization was developed. After filtration with PES3 membrane, an activated charcoal was used to remove the small proteins and pigments in the UF permeate. As a result, 91.4% of DC12 recovery, 94.7% of decolorization ratio and 84.8% of deproteinization ratio were obtained by such two-stage strategy. These results would serve as a valuable guide for process design and practical operation in subsequent industrial application.
机译：α，ω-二羧酸（DC）是具有不同链长的通用化学中间体。对于DC的生物合成，为了通过结晶获得高纯度的产品，需要去除发酵液中的色素和蛋白质。然而，发现在纯化过程中脱色/脱蛋白率与DC回收率之间存在折衷，这阻碍了发酵产生DC。当用超滤（UF）处理α，ω-十二碳二酸（DC12）肉汤时，通过PES 3膜可实现93.4％的DC12回收率，80.5％的脱色率和61.7％的脱蛋白率。但是，当需要高DC12渗透时，膜技术无法有效地保留低分子量的颜料或蛋白质。同时，所选的活性炭或大孔树脂对于本系统不是良好的吸附剂。此外，开发了用于脱色和脱蛋白的综合方法。用PES3膜过滤后，使用活性炭除去UF渗透物中的小蛋白质和色素。结果，通过这种两阶段策略获得了91.4％的DC12回收率，94.7％的脱色率和84.8％的脱蛋白率。这些结果将为后续工业应用中的工艺设计和实际操作提供有价值的指导。

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