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首页> 外文期刊>Bioprocess and Biosystems Engineering >Cloning, expression, and characterization of an alkaline protease, AprV, from Vibrio sp. DA1-1
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Cloning, expression, and characterization of an alkaline protease, AprV, from Vibrio sp. DA1-1

机译：来自弧菌属的碱性蛋白酶AprV的克隆，表达和鉴定。 DA1-1

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摘要

A novel alkaline protease (named AprV) gene from Vibrio sp. DA1-1 was cloned and expressed in Escherichia coli BL21 (DE3) pLysS. The sequence analysis showed the highest homology of 68% with the characterized protease from Alkalimonas collagenimarina AC40(T). The recombinant AprV was purified with the molecular weight of 28 kDa. The optimum temperature and pH were determined to be 55 degrees C and 10.0, respectively. The enzyme activity was slightly enhanced by Ca2+, Mg2+, Zn2+, Ba2+, and, however, was highly inhibited by Sn2+ and EDTA. The AprV was stable in the presence of some surfactants and oxidizing agents, such as 1% Tween 20-80, 1% JFC-2, and 5% JFC-2. Casein was found to be the ideal substrate with specific activity of 1139 U/mg. Moreover, we found that AprV (10,000 U), together with commercial detergent, could completely remove the blood on the cotton. Furthermore, AprV also demonstrated dehairing activity on goat and bull skin. These results indicated that the alkaline protease AprV might be a potential candidate for applications in the detergent and leather industries.
机译：一种来自弧菌的新型碱性蛋白酶（命名为AprV）基因。 DA1-1被克隆并在大肠杆菌BL21（DE3）pLysS中表达。序列分析显示与来自碱性胶原胶原AC40（T）的特征性蛋白酶的最高同源性为68％。以28kDa的分子量纯化重组AprV。最佳温度和pH分别确定为55摄氏度和10.0。 Ca2 +，Mg2 +，Zn2 +，Ba2 +略微增强了酶的活性，但是Sn2 +和EDTA却高度抑制了酶的活性。 AprV在某些表面活性剂和氧化剂（例如1％Tween 20-80、1％JFC-2和5％J​​FC-2）的存在下稳定。发现酪蛋白是具有1139U / mg的比活性的理想底物。此外，我们发现AprV（10,000 U）与商业洗涤剂一起可以完全清除棉花上的血液。此外，AprV还显示了对山羊和公牛皮肤的脱毛活性。这些结果表明碱性蛋白酶AprV可能是在洗涤剂和皮革工业中应用的潜在候选者。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2018年第10期|1437-1447|共11页
  • 作者

    Chen Xiujuan; Zhou Cheng; Xue Yanfen; Shi Jinsong; Ma Yanhe;

  • 作者单位

    Jiangnan Univ, Sch Pharmaceut Sci, 1800 Lihu Ave, Wuxi 214122, Peoples R China;

    Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, 1 West Beichen Rd, Beijing 100101, Peoples R China;

    Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, 1 West Beichen Rd, Beijing 100101, Peoples R China;

    Jiangnan Univ, Sch Pharmaceut Sci, 1800 Lihu Ave, Wuxi 214122, Peoples R China;

    Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, 1 West Beichen Rd, Beijing 100101, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Protease; Vibrio; Detergent; Dehairing; Surfactant stable;

    机译：蛋白酶;弧菌;洗涤剂;脱毛;表面活性剂稳定剂;

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