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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enhanced e-poly-L-lysine production by inducing double antibiotic-resistant mutations in Streptomyces albulus
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Enhanced e-poly-L-lysine production by inducing double antibiotic-resistant mutations in Streptomyces albulus

机译：通过诱导白色链霉菌中双重抗药性突变来增强e-poly-L-赖氨酸的生产

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摘要

epsilon-Poly-L-lysine (epsilon-PL), as a food additive, has been widely used in many countries. However, its production still needs to be improved. We successfully enhanced epsilon-PL production of Streptomyces albulus FEEL-1 by introducing mutations related to antibiotics, such as streptomycin, gentamicin, and rifampin. Single-and doubl-epsilon-resistant mutants (S-88 and SG-31) were finally screened with the improved epsilon-PL productions of 2.81 and 3.83 g/L, 1.75-to 2.39-fold compared with that of initial strain FEEL-1. Then, the performances of mutants S-88 and SG-31 were compared with the parent strain FEEL-1 in a 5-L bioreactor under the optimal condition for epsilon-PL production. After 174-h fed-batch fermentation, the epsilon-PL production and productivity of hyper-strain SG-31 reached the maximum of 59.50 g/L and 8.21 g/L/day, respectively, which was 138 and 105% higher than that of FEEL-1. Analysis of streptomycin-resistant mutants demonstrated that a point mutation occurred in rpsL gene (encoding the ribosomal protein S12). These single and double mutants displayed remarkable increases of the activities and transcriptional levels of key enzymes in epsilon-PL biosynthesis pathway, which may be responsible for the enhanced mycelia viability, respiratory activity, and epsilon-PL productions of SG-31. These results showed that the new breeding method, called ribosome engineering, could be a novel and effective breeding strategy for the evolution of epsilon-PL-producing strains.
机译：作为食品添加剂的epsilon-Poly-L-赖氨酸（epsilon-PL）已在许多国家广泛使用。但是，其产量仍需改进。通过引入与抗生素有关的突变，例如链霉素，庆大霉素和利福平，我们成功增强了小球藻链霉菌FEEL-1的ε-PL产生。最终筛选出具有单抗和双螺旋抗性的突变体（S-88和SG-31），其ε-PL产量提高了2.81和3.83 g / L，是初始菌株FEEL-的1.75-2.39倍。 1。然后，在产生ε-PL的最佳条件下，在5-L生物反应器中将突变体S-88和SG-31的性能与亲本菌株FEEL-1的性能进行了比较。分批补料发酵174h后，超级菌株SG-31的epsilon-PL产量和生产率分别达到最高59.50 g / L和8.21 g / L / day，分别比其高138和105％。的FEEL-1。对链霉素抗性突变体的分析表明，rpsL基因（编码核糖体蛋白S12）中发生了点突变。这些单突变体和双突变体显示出ε-PL生物合成途径中关键酶的活性和转录水平的显着增加，这可能是SG-31菌丝活力，呼吸活性和ε-PL产生增强的原因。这些结果表明，称为核糖体工程的新育种方法可能是产生ε-PL的菌株进化的一种新颖而有效的育种策略。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2017年第2期|271-283|共13页
  • 作者

    Wang Liang; Chen Xusheng; Wu Guangyao; Li Shu; Zeng Xin; Ren Xidong; Tang Lei; Mao Zhonggui;

  • 作者单位

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China;

    Xiwang Grp Co Ltd, Zouping 256209, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Streptomyces albulus FEEL-1; Key enzyme activities; qRT-PCR; Ribosome engineering; Double drug-resistant mutation;

    机译：链霉菌FEEL-1;关键酶活性;qRT-PCR;核糖体工程;双重耐药突变;

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