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首页> 外文期刊>Bioprocess and Biosystems Engineering >Chitosan hydrogel microspheres: an effective covalent matrix for crosslinking of soluble dextranase to increase stability and recycling efficiency
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Chitosan hydrogel microspheres: an effective covalent matrix for crosslinking of soluble dextranase to increase stability and recycling efficiency

机译：壳聚糖水凝胶微球：有效的共价基质，可交联可溶性葡聚糖酶以增加稳定性和回收效率

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摘要

Dextranase is a unique biocatalyst that has high specificity and stereo-selectivity towards a complex biopolymer known as dextran. Dextranase has wide industrial application, but most of the time harsh environmental conditions adversely affect the functionality and stability of the enzyme. To overcome this issue, a covalent cross-linking immobilization method was adapted in the current study utilizing a nontoxic and biocompatible matrix known as chitosan. Chitosan hydrogel microspheres were synthesized using chitosan which exhibited noteworthy physical and mechanical strength. After treatment with glutaraldehyde, chitosan hydrogel microspheres were used for immobilization of dextranase. The kinetic characteristics of immobilized dextranase were compared with that of the soluble enzyme. A shift in optimum pH and temperature from 7.0 to 7.5 and 50 to 60 A degrees C was observed after immobilization, respectively. Recycling efficiency, thermal stability, and activation energy distinctly improved after immobilization, whereas anchoring of substrate at the active site of the soluble dextranase exhibited an increase in K (m) with no change in V (max) after crosslinking. This technique involves the reduction in the size of carrier molecules (microspheres) that provide a larger surface area for improved immobilization efficiency. Therefore, it is concluded that increased stability and reusability of this immobilized biocatalyst makes it a promising aspirant for the utilization at commercial level.
机译：葡聚糖酶是独特的生物催化剂，对称为葡聚糖的复杂生物聚合物具有高特异性和立体选择性。葡聚糖酶具有广泛的工业应用，但是在大多数情况下，恶劣的环境条件会对酶的功能和稳定性产生不利影响。为了克服这个问题，目前的研究采用了一种无毒且生物相容的基质，即壳聚糖，对共价交联固定化方法进行了调整。壳聚糖水凝胶微球是使用壳聚糖合成的，其具有显着的物理和机械强度。用戊二醛处理后，使用壳聚糖水凝胶微球固定葡聚糖酶。比较了固定化葡聚糖酶和可溶性酶的动力学特性。固定后，分别观察到最佳pH和温度分别从7.0到7.5和50到60 A的变化。固定后，回收效率，热稳定性和活化能明显提高，而将底物锚定在可溶性葡聚糖酶的活性位点上，则交联后K（m）增大，而V（max）不变。该技术涉及减小载体分子（微球）的尺寸，该载体分子（微球）提供更大的表面积以提高固定效率。因此，可以得出结论，这种固定化生物催化剂的稳定性和可重复使用性的提高使其成为工业化应用的有希望的应用前景。

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