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首页> 外文期刊>Bioprocess and Biosystems Engineering >Bioethanol production from Gracilaria verrucosa using Saccharomyces cerevisiae adapted to NaCl or galactose
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Bioethanol production from Gracilaria verrucosa using Saccharomyces cerevisiae adapted to NaCl or galactose

机译：使用适应于NaCl或半乳糖的酿酒酵母从拟南芥（Gracilaria verrucosa）生产生物乙醇

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摘要

This study examined the pretreatment, enzymatic saccharification, and fermentation of the red macroalgae Gracilaria verrucosa using adapted saccharomyces cerevisiae to galactose or NaCl for the increase of bioethanol yield. Pretreatment with thermal acid hydrolysis to obtain galactose was carried out with 11.7% (w/v) seaweed slurry and 373 mM H2SO4 at 121 A degrees C for 59 min. Glucose was obtained from enzymatic hydrolysis. Enzymatic saccharification was performed with a mixture of 16 U/mL Celluclast 1.5L and Viscozyme L at 45 A degrees C for 48 h. Ethanol fermentation in 11.7% (w/v) seaweed hydrolysate was carried out using Saccharomyces cerevisiae KCTC 1126 adapted or non-adapted to high concentrations of galactose or NaCl. When non-adapted S. cerevisiae KCTC 1126 was used, the ethanol productivity was 0.09 g/(Lh) with an ethanol yield of 0.25. Ethanol productivity of 0.16 and 0.19 g/(Lh) with ethanol yields of 0.43 and 0.48 was obtained using S. cerevisiae KCTC 1126 adapted to high concentrations of galactose and NaCl, respectively. Adaptation of S. cerevisiae KCTC 1126 to galactose or NaCl increased the ethanol yield via adaptive evolution of the yeast.
机译：这项研究检查了红色大型藻类拟南芥的预处理，酶促糖化和发酵过程，使用了酿酒酵母对半乳糖或NaCl进行了发酵，以提高生物乙醇的产量。用热酸水解进行预处理以获得半乳糖，是在11.21 A摄氏度下用11.7％（w / v）的海藻浆液和373 mM H2SO4进行59分钟。葡萄糖是通过酶水解获得的。在45 A摄氏度下，用16 U / mL Celluclast 1.5L和Viscozyme L的混合物进行酶促糖化48小时。使用适合或不适合高浓度半乳糖或NaCl的酿酒酵母KCTC 1126在11.7％（w / v）海藻水解物中进行乙醇发酵。当使用非适应性酿酒酵母KCTC 1126时，乙醇生产率为0.09g /（Lh），乙醇产率为0.25。使用分别适用于高浓度半乳糖和NaCl的酿酒酵母KCTC 1126，可获得0.16和0.19 g /（Lh）的乙醇产率以及0.43和0.48的乙醇产率。酿酒酵母KCTC 1126对半乳糖或NaCl的适应通过酵母的适应性进化提高了乙醇产量。

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