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Enhanced production of para-hydroxybenzoic acid by genetically engineered Saccharomyces cerevisiae

机译：基因工程酿酒酵母提高了对羟基苯甲酸的产量

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摘要

Saccharomyces cerevisiae is a popular organism for metabolic engineering; however, studies aiming at over-production of bio-replacement precursors for the chemical industry often fail to overcome proof-of-concept stage. When intending to show real industrial attractiveness, the challenge is twofold: formation of the target compound must be increased, while minimizing the formation of side and by-products to maximize titer, rate and yield. To tackle these, the metabolism of the organism, as well as the parameters of the process, need to be optimized. Addressing both we show that S. cerevisiae is well-suited for over-production of aromatic compounds, which are valuable in chemical industry and are particularly useful in space technology. Specifically, a strain engineered to accumulate chorismate was optimized for formation of para-hydroxybenzoic acid. Then a fed-batch bioreactor process was developed, which delivered a final titer of 2.9 g/L, a maximum rate of 18.625 mg(pHBA)/(g(CDW) x h) and carbon-yields of up to 3.1 mg(pHBA)/g(glucose).
机译：酿酒酵母是用于代谢工程的流行生物。但是，针对化学工业中生物替代前体的超量生产的研究通常无法克服概念验证阶段。当打算表现出真正的工业吸引力时，挑战是双重的：必须增加目标化合物的形成，同时最小化副产物和副产物的形成以最大化滴度，速率和产率。为了解决这些问题，需要优化生物的代谢以及过程的参数。针对这两个方面，我们表明酿酒酵母非常适合过量生产芳香族化合物，这在化学工业中非常有价值，在航天技术中特别有用。具体地，优化工程化以积累分支酸盐的菌株以形成对羟基苯甲酸。然后开发了分批补料生物反应器工艺，最终滴定度为2.9 g / L，最大滴定度为18.625 mg（pHBA）/（g（CDW）xh），碳收率高达3.1 mg（pHBA） / g（葡萄糖）。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2017年第8期|1283-1289|共7页
  • 作者

    Averesch Nils J. H.; Prima Alex; Kroemer Jens O.;

  • 作者单位

    Univ Queensland, Ctr Microbial Electrochem Syst CEMES, Brisbane, Qld, Australia|Univ Queensland, AWMC, Brisbane, Qld, Australia|NASA, Ames Res Ctr, Univ Space Res Assoc, Mountain View, CA 94035 USA;

    Univ Queensland, Ctr Microbial Electrochem Syst CEMES, Brisbane, Qld, Australia|Univ Queensland, AWMC, Brisbane, Qld, Australia|Tech Univ Dortmund, Dept Tech Biochem, Dortmund, Germany;

    Univ Queensland, Ctr Microbial Electrochem Syst CEMES, Brisbane, Qld, Australia|Univ Queensland, AWMC, Brisbane, Qld, Australia|Helmholtz Ctr Environm Res, Dept Solar Mat, Leipzig, Germany;

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  • 正文语种 eng
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  • 关键词

    Para-hydroxybenzoic acid; Shikimate pathway; Saccharomyces cerevisiae; Fed-batch;

    机译：对羟基苯甲酸Shi酸酯途径酿酒酵母补料分批;

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