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首页> 外文期刊>Bioprocess and Biosystems Engineering >Pretreatment of fibrous biomass and growth of biosurfactant-producing Bacillus subtilis on biomass-derived fermentable sugars
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Pretreatment of fibrous biomass and growth of biosurfactant-producing Bacillus subtilis on biomass-derived fermentable sugars

机译：纤维状生物质的预处理以及生物质衍生的发酵糖上生物活性剂生产枯草芽孢杆菌的生长

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Pretreatment of six fibrous biomasses (switchgrass, alfalfa, soy hulls, soy fiber, DDGS and Baggase) and subsequent hydrolysis using cellulolytic enzymes at a 2.5 % (v/v) and 5 % (v/v) loading 2.5 (v/v) and 5 % (v/v) loading was compared for higher amounts of sugars released. Soaking of biomasses of switchgrass, alfalfa, soy hulls and bagasse in 15 % w/w ammonia was optimal at 60 A degrees C for 12 h, followed by enzymatic hydrolysis, yielding 72, 70, 80 and 75 % carbohydrate conversions, respectively. However, soaking in ammonia was not needed for soy fiber and DDGS as these contained very little lignin. Ultrasonication for 3 min @ 100 % amplitude (170 A mu M) was found to be optimal for soy fiber and DDGS from which 77 and 83 % carbohydrate conversion, respectively, was obtained following enzyme treatment at 5 % (w/v) enzyme. The sugars released by enzymatic hydrolysis of pretreated biomass were utilized as an energy source by Bacillus subtilis in fermentation media at 2 % (w/v) of concentration. In shake flask trials, cell growth was 15-20 % higher on hydrolysates of ammonia-treated switchgrass and alfalfa vs. glucose-based control media due to the presence of a wider range of monomeric sugars (glucose, xylose, arabinose, mannose and galactose). In contrast, growth was less on soy hull hydrolysates prepared with ammonia pretreatment.
机译：预处理六种纤维状生物量（柳枝,、苜蓿，大豆皮，大豆纤维，DDGS和Baggase），随后使用纤维素分解酶以2.5％（v / v）和5％（v / v）负载2.5（v / v）进行水解比较了5％（v / v）的装载量，以释放更多的糖。柳枝,、苜蓿，大豆壳和甘蔗渣的生物量在15°w / w的氨中浸泡的最佳时间是在60 A的温度下浸泡12 h，然后进行酶水解，分别产生72％，70％，80％和75％的碳水化合物转化率。但是，大豆纤维和DDGS不需要浸泡在氨中，因为它们所含的木质素很少。发现超声处理在振幅为100％（170 AμM）的情况下超声处理3分钟是大豆纤维和DDGS的最佳选择，在5％（w / v）酶处理后，分别从其中获得77％和83％的碳水化合物转化率。酶解水解预处理过的生物质释放的糖被枯草芽孢杆菌用作发酵培养基中浓度为2％（w / v）的能源。在摇瓶试验中，由于存在更多种类的单体糖（葡萄糖，木糖，阿拉伯糖，甘露糖和半乳糖），与基于葡萄糖的对照培养基相比，经氨处理的柳枝and和苜蓿水解产物的细胞生长高15-20％。 ）。相反，用氨预处理制备的大豆壳水解产物的生长较少。

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