...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Efficient production of alpha-ketoglutarate in the gdh deleted Corynebacterium glutamicum by novel double-phase pH and biotin control strategy
【24h】

Efficient production of alpha-ketoglutarate in the gdh deleted Corynebacterium glutamicum by novel double-phase pH and biotin control strategy

机译：通过新型双相pH和生物素控制策略，在缺失gdh的谷氨酸棒状杆菌中高效生产α-酮戊二酸

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Production of L-glutamate using a biotin-deficient strain of Corynebacterium glutamicum has a long history. The process is achieved by controlling biotin at suboptimal dose in the initial fermentation medium, meanwhile feeding NH4OH to adjust pH so that alpha-ketoglutarate (alpha-KG) can be converted to L-glutamate. In this study, we deleted glutamate dehydrogenase (gdh1 and gdh2) of C. glutamicum GKG-047, an L-glutamate over-producing strain, to produce alpha-KG that is the direct pre-cursor of L-glutamate. Based on the method of L-glutamate fermentation, we developed a novel double-phase pH and biotin control strategy for alpha-KG production. Specifically, NH4OH was added to adjust the pH at the bacterial growth stage and NaOH was used when the cells began to produce acid; besides adding an appropriate amount of biotin in the initial medium, certain amount of additional biotin was supplemented at the middle stage of fermentation to maintain a high cell viability and promote the carbon fixation to the flux of alpha-KG production. Under this control strategy, 45.6 g/L alpha-KG accumulated after 30-h fermentation in a 7.5-L fermentor and the productivity and yield achieved were 1.52 g/L/h and 0.42 g/g, respectively.
机译：使用缺乏生物素的谷氨酸棒杆菌菌株生产L-谷氨酸具有悠久的历史。通过在初始发酵培养基中控制次最佳剂量的生物素，同时加入NH4OH来调节pH，从而使α-酮戊二酸酯（alpha-KG）转化为L-谷氨酸，可以实现该过程。在这项研究中，我们删除了谷氨酸棒杆菌GKG-047（一种过量生产L-谷氨酸的菌株）的谷氨酸脱氢酶（gdh1和gdh2），以生产α-KG，它是L-谷氨酸的直接前体。基于L-谷氨酸发酵的方法，我们开发了一种新型的双相pH和生物素控制策略来生产α-KG。具体来说，在细菌生长阶段加入NH4OH调节pH值，当细胞开始产生酸时使用NaOH。除了在初始培养基中添加适量的生物素外，发酵的中期还补充了一定量的其他生物素，以维持较高的细胞活力并促进碳固定在α-KG产生的通量上。在这种控制策略下，在7.5 L的发酵罐中发酵30小时后，累积了45.6 g / L的α-KG，生产率和产量分别为1.52 g / L / h和0.42 g / g。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2016年第6期|967-976|共10页
  • 作者

    Li Yanjun; Sun Lanchao; Feng Jia; Wu Ruifang; Xu Qingyang; Zhang Chenglin; Chen Ning; Xie Xixian;

  • 作者单位

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China|Natl & Local United Engn Lab Metab Control Fermen, Tianjin 300457, Peoples R China|Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China|Natl & Local United Engn Lab Metab Control Fermen, Tianjin 300457, Peoples R China|Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China|Natl & Local United Engn Lab Metab Control Fermen, Tianjin 300457, Peoples R China|Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China|Natl & Local United Engn Lab Metab Control Fermen, Tianjin 300457, Peoples R China|Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Tianjin 300457, Peoples R China;

    Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China|Natl & Local United Engn Lab Metab Control Fermen, Tianjin 300457, Peoples R China|Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Tianjin 300457, Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Corynebacterium glutamicum; alpha-Ketoglutarate; L-Glutamate; Biotin; pH;

    机译：谷氨酸棒杆菌;α-酮戊二酸;L-谷氨酸;生物素;pH;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号