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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enhanced citric acid production by a yeast Yarrowia lipolytica over-expressing a pyruvate carboxylase gene
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Enhanced citric acid production by a yeast Yarrowia lipolytica over-expressing a pyruvate carboxylase gene

机译：过表达丙酮酸羧化酶基因的酵母解脂耶氏酵母提高了柠檬酸的产生

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摘要

In this study, after the expression of a pyruvate carboxylase gene (PYC) cloned from Meyerozyma guilliermondii in a marine-derived yeast Yarrowia lipolytica SWJ-1b, a transformant PG86 obtained had much higher PYC activity than Y. lipolytica SWJ-1b. At the same time, the PYC gene expression and citric acid (CA) production by the transformant PG86 were also greatly enhanced. When glucose concentration in the medium was 60.0 g L-1, CA concentration formed by the transformant PG86 was 34.02 g L-1, leading to a CA yield of 0.57 g g(-1) of glucose. During a 10-L fed-batch fermentation, the final concentration of CA was 101.0 +/- A 1.3 g L-1, the yield was 0.89 g g(-1) of glucose, the productivity was 0.42 g L-1 h(-1) and only 5.93 g L-1 reducing sugar was left in the fermented medium within 240 h of the fed-batch fermentation. HPLC analysis showed that most of the fermentation products were CA.
机译：在这项研究中，在海生酵母解脂耶氏酵母SWJ-1b中表达了从古细菌耶氏酵母克隆的丙酮酸羧化酶基因（PYC）的表达，所获得的转化体PG86比解脂耶氏酵母SWJ-1b具有更高的PYC活性。同时，转化体PG86的PYC基因表达和柠檬酸（CA）产生也大大增强。当培养基中的葡萄糖浓度为60.0 g L-1时，转化体PG86形成的CA浓度为34.02 g L-1，导致CA产量为0.57 g g（-1）葡萄糖。在10 L分批补料发酵中，CA的终浓度为101.0 +/- A 1.3 g L-1，产量为0.89 gg（-1）葡萄糖，生产率为0.42 g L-1 h（- 1）在分批补料发酵的240小时内，仅5.93 g L-1还原糖留在发酵培养基中。 HPLC分析表明，大多数发酵产物是CA。

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