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Enhanced acid tolerance of Rhizopus oryzae during fumaric acid production

机译：富马酸生产过程中米根霉的耐酸性增强

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摘要

Ensuring a suitable pH in the culture broth is a major problem in microorganism-assisted industrial fermentation of organic acids. To address this issue, we investigated the physiological changes in Rhizopus oryzae at different extracellular pH levels and attempted to solve the issue of cell shortage under low pH conditions. We compared various parameters, such as membrane fatty acids' composition, intracellular pH, and adenosine triphosphate (ATP) concentration. It was found that the shortage of intracellular ATP might be the main reason for the low rate of fumaric acid production by R. oryzae under low pH conditions. When 1 g/l citrate was added to the culture medium at pH 3.0, the intracellular ATP concentration increased from 0.4 to 0.7 μmol/mg, and the fumaric acid titer was enhanced by 63 % compared with the control (pH 3.0 without citrate addition). The final fumaric acid concentration at pH 3.0 reached 21.9 g/l after 96 h of fermentation. This strategy is simple and feasible for industrial fumaric acid production under low pH conditions.
机译：在微生物辅助的有机酸工业发酵中，确保培养液中合适的pH是主要问题。为了解决这个问题，我们研究了米根霉在不同细胞外pH值下的生理变化，并试图解决低pH条件下细胞短缺的问题。我们比较了各种参数，例如膜脂肪酸的组成，细胞内pH值和三磷酸腺苷（ATP）浓度。已发现，细胞内ATP的缺乏可能是稻在低pH条件下稻瘟病菌富马酸产生率低的主要原因。当在pH 3.0的培养基中添加1 g / l柠檬酸盐时，细胞内ATP浓度从0.4增加到0.7μmol/ mg，富马酸滴度比对照（pH 3.0，不添加柠檬酸盐）提高63％。 。发酵96小时后，pH 3.0下的富马酸终浓度达到21.9 g / l。对于在低pH条件下生产工业富马酸而言，该策略简单可行。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2015年第2期|323-328|共6页
  • 作者

    Ying Liu; Chunwei Lv; Qing Xu; Shuang Li; He Huang; Pingkai Ouyang;

  • 作者单位

    State Key Laboratory of Material-oriented Chemical Engineering College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu Road, Nanjing 211816, China,Science and Technology Development Department, Sinopec Corporation, No. 22 Chaoyangmen North Street, Changyang District, Beijing 100728, China;

    State Key Laboratory of Material-oriented Chemical Engineering College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu Road, Nanjing 211816, China;

    State Key Laboratory of Material-oriented Chemical Engineering College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu Road, Nanjing 211816, China;

    State Key Laboratory of Material-oriented Chemical Engineering College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu Road, Nanjing 211816, China;

    State Key Laboratory of Material-oriented Chemical Engineering College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu Road, Nanjing 211816, China;

    State Key Laboratory of Material-oriented Chemical Engineering College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu Road, Nanjing 211816, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Rhizopus oryzae; Fumaric acid; Low pH fermentation; Acid tolerance; ATP;

    机译：米根霉;富马酸;低pH发酵;耐酸ATP;

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