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首页> 外文期刊>Bioprocess and Biosystems Engineering >Acidic pH shock induced overproduction of epsilon-poly-l-lysine in fed-batch fermentation by Streptomyces sp M-Z18 from agro-industrial by-products
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Acidic pH shock induced overproduction of epsilon-poly-l-lysine in fed-batch fermentation by Streptomyces sp M-Z18 from agro-industrial by-products

机译：酸性pH休克诱导链霉菌sp M-Z18在农用工业副产物的分批补料发酵中过量生产epsilon-poly-l-赖氨酸

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摘要

epsilon-Poly-l-lysine (epsilon-PL) is produced by Streptomyces as a secondary metabolite with wide industrial applications, but its production still needs to be further enhanced. Environmental stress is an important approach for the promotion of secondary metabolites production by Streptomyces. In this study, the effect of acidic pH shock on enhancing epsilon-PL production by Streptomyces sp. M-Z18 was investigated in a 5-L fermenter. Based on the evaluation of acidic pH shock on mycelia metabolic activity and shock parameters optimization, an integrated pH-shock strategy was developed as follows: pre-acid-shock adaption at pH 5.0 to alleviate the damage caused by the followed pH shock, and then acidic pH shock at 3.0 for 12 h (including pH decline from 4.0 to 3.0) to positively regulate mycelia metabolic activity, finally restoring pH to 4.0 to provide optimal condition for epsilon-PL production. After 192 h of fed-batch fermentation, the maximum epsilon-PL production and productivity reached 54.70 g/L and 6.84 g/L/day, respectively, which were 52.50 % higher than those of control without pH shock. These results demonstrated that acidic pH shock is an efficient approach for improving epsilon-PL production. The information obtained should be useful for epsilon-PL production by other Streptomyces.
机译：链霉菌产生的ε-聚-1-赖氨酸（epsilon-PL）是次生代谢产物，具有广泛的工业应用，但其产量仍需进一步提高。环境胁迫是促进链霉菌产生次生代谢产物的重要方法。在这项研究中，酸性pH休克对链霉菌spepsonon-PL生产的影响。在5-L发酵罐中研究了M-Z18。在评估酸性pH休克对菌丝体代谢活性和优化休克参数的基础上，开发了一种综合的pH休克策略：在pH 5.0时预先进行酸休克适应以减轻随后的pH休克所造成的损害，然后在3.0的酸性pH冲击下处理12小时（包括pH从4.0降低到3.0），以积极调节菌丝体的代谢活性，最后将pH恢复至4.0，从而为ε-PL生产提供最佳条件。分批补料发酵192小时后，最大的epsilon-PL产量和生产率分别达到54.70 g / L和6.84 g / L / day，比没有pH冲击的对照高52.50％。这些结果表明，酸性pH冲击是提高ε-PL产量的有效方法。获得的信息对于其他链霉菌的ε-PL的生产应该是有用的。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2015年第6期|1113-1125|共13页
  • 作者

    Ren Xi-Dong; Chen Xu-Sheng; Zeng Xin; Wang Liang; Tang Lei; Mao Zhong-Gui;

  • 作者单位

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Jiangsu, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Jiangsu, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    epsilon-Poly-L-lysine; Streptomyces sp M-Z18; Acidic pH shock; Acid tolerance response; Viability;

    机译：ε-聚-L-赖氨酸;链霉菌M-Z18;酸性pH冲击;耐酸反应;生存力;

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