...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Identification of aldehyde reductase catalyzing the terminal step for conversion of xylose to butanetriol in engineered Escherichia coli
【24h】

Identification of aldehyde reductase catalyzing the terminal step for conversion of xylose to butanetriol in engineered Escherichia coli

机译：鉴定醛还原酶催化工程化大肠杆菌中木糖转化为丁三醇的最终步骤

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Biosynthetic pathways for the production of biofuels often rely on inherent aldehyde reductases (ALRs) of the microbial host. These native ALRs play vital roles in the success of the microbial production of 1,3-propanediol, 1,4-butanediol, and isobutanol. In the present study, the main ALR for 1,2,4-butanetriol (BT) production in Escherichia coli was identified. Results of real-time PCR analysis for ALRs in EWBT305 revealed the increased expression of adhP, fucO, adhE, and yqhD genes during BT production. The highest increase of expression was observed up to four times in yqhD. Singular deletion of adhP, fucO, or adhE gene showed marginal differences in BT production compared to that of the parent strain, EWBT305. Remarkably, yqhD gene deletion (KBTA4 strain) almost completely abolished BT production while its re-introduction (wild-type gene with its native promoter) on a low copy plasmid restored 75 % of BT production (KBTA4-2 strain). This suggests that yqhD gene is the main ALR of the BT pathway. In addition, KBTA4 showed almost no NADPH-dependent ALR activity, but was also restored upon re-introduction of the yqhD gene (KBTA4-2 strain). Therefore, the required ALR activity to complete the BT pathway was mainly contributed by YqhD. Increased gene expression and promiscuity of YqhD were both found essential factors to render YqhD as the key ALR for the BT pathway.
机译：生产生物燃料的生物合成途径通常依赖于微生物宿主固有的醛还原酶（ALR）。这些天然ALR在成功生产1,3-丙二醇，1,4-丁二醇和异丁醇的微生物中起着至关重要的作用。在本研究中，确定了在大肠杆菌中生产1,2,4-丁三醇（BT）的主要ALR。 EWBT305中ALR的实时PCR分析结果显示，BT生产过程中adhP，fucO，adhE和yqhD基因表达增加。在yqhD中观察到最高的表达增加，达到四倍。与亲本菌株EWBT305相比，单数缺失adhP，fucO或adhE基因显示出BT产生的边际差异。值得注意的是，yqhD基因缺失（KBTA4株）几乎完全消除了BT的产生，而其在低拷贝质粒上的重新导入（带有其天然启动子的野生型基因）则恢复了BT产生的75％（KBTA4-2株）。这表明yqhD基因是BT途径的主要ALR。此外，KBTA4几乎没有显示NADPH依赖性的ALR活性，但在重新引入yqhD基因（KBTA4-2株）后也得以恢复。因此，完成BT通路所需的ALR活性主要由YqhD贡献。发现基因表达的增加和YqhD的滥交都是使YqhD成为BT途径的关键ALR的重要因素。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2015年第9期|1761-1772|共12页
  • 作者

    Valdehuesa Kris Nino G.; Lee Won-Keun; Ramos Kristine Rose M.; Cabulong Rhudith B.; Choi JiSoo; Liu Huaiwei; Nisola Grace M.; Chung Wook-Jin;

  • 作者单位

    Myongji Univ, Dept Energy Sci & Technol, Energy & Environm Fus Technol Ctr E2FTC, Yongin, Gyeonggi Do, South Korea;

    Myongji Univ, Div Biosci & Bioinformat, Yongin, Gyeonggi Do, South Korea;

    Myongji Univ, Dept Energy Sci & Technol, Energy & Environm Fus Technol Ctr E2FTC, Yongin, Gyeonggi Do, South Korea;

    Myongji Univ, Dept Energy Sci & Technol, Energy & Environm Fus Technol Ctr E2FTC, Yongin, Gyeonggi Do, South Korea;

    Myongji Univ, Dept Energy Sci & Technol, Energy & Environm Fus Technol Ctr E2FTC, Yongin, Gyeonggi Do, South Korea;

    Myongji Univ, Dept Energy Sci & Technol, Energy & Environm Fus Technol Ctr E2FTC, Yongin, Gyeonggi Do, South Korea;

    Myongji Univ, Dept Energy Sci & Technol, Energy & Environm Fus Technol Ctr E2FTC, Yongin, Gyeonggi Do, South Korea;

    Myongji Univ, Dept Energy Sci & Technol, Energy & Environm Fus Technol Ctr E2FTC, Yongin, Gyeonggi Do, South Korea;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    1,2,4-butanetriol; Escherichia coli; YqhD; Aldehyde reductase; Xylose; Dahms pathway;

    机译：1,2,4-丁三醇;大肠杆菌;YqhD;醛还原酶;木糖;Dahms途径;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号