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首页> 外文期刊>Bioprocess and Biosystems Engineering >Daptomycin antibiotic production processes in fed-batch fermentation by Streptomyces roseosporus NRRL11379 with precursor effect and medium optimization
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Daptomycin antibiotic production processes in fed-batch fermentation by Streptomyces roseosporus NRRL11379 with precursor effect and medium optimization

机译:玫瑰孢链霉菌NRRL11379分批补料发酵达托霉素抗生素的生产过程

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摘要

Sodium decanoate was first found to be an effective precursor for synthesis of daptomycin from Streptomyces roseosporus NRRL11379 which was increased to 71.55-fold, compared with decanoic acid. The optimal flow rate of precursor was at 600 mg/(L day) after 48 h fermentation. From protein analysis via SDS-PAGE and identification of Tandem MS/MS afterwards, it deciphered that guanosine pentaphosphate synthetase, PNPase, tripeptidylamino peptidase primarily dealing with daptomycin synthesis. By applying Taguchi's L16 in culture optimization, the best yield was obtained from the medium with 60 g/L dextrin, 10 g/L dextrose, 1.0 g/L molasses, and 8 g/L yeast extract, respectively. The fed-batch fermentation, applied with feedback control of dextrin, stimulated the production up to 812 mg/L at 288 h. To our best knowledge, the daptomycin production in this study is significantly higher than that in previous studies and can make it more widely used in pharmaceutical industry.
机译:首先发现癸酸钠是从玫瑰链霉菌NRRL11379合成达托霉素的有效前体,与癸酸相比,它增加了71.55倍。发酵48小时后,前体的最佳流速为600 mg /(L天)。从通过SDS-PAGE进行的蛋白质分析和随后的串联MS / MS鉴定,可以看出鸟苷五磷酸合成酶,PNPase,三肽氨基肽酶主要处理达托霉素的合成。通过在培养优化中应用田口氏L16,可分别从含有60 g / L糊精,10 g / L葡萄糖,1.0 g / L糖蜜和8 g / L酵母提取物的培养基中获得最佳产量。分批补料发酵,采用糊精的反馈控制,在288 h时将产量提高到812 mg / L。据我们所知,达托霉素的产量明显高于以前的研究,可以使其在制药工业中得到更广泛的应用。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第3期|415-423|共9页
  • 作者单位

    Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China,The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China;

    Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;

    Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;

    Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China,The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China;

    Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China,The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Daptomycin antibiotic; Streptomyces roseosporus; Fed-batch fermentation; Optimization; Sodium decanoate;

    机译:达托霉素抗生素;玫瑰孢链霉菌;分批补料发酵;优化;癸酸钠;

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