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首页> 外文期刊>Bioprocess and Biosystems Engineering >Effect of bioconversion conditions on vanillin production by Amycolatopsis sp. ATCC 39116 through an analysis of competing by-product formation
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Effect of bioconversion conditions on vanillin production by Amycolatopsis sp. ATCC 39116 through an analysis of competing by-product formation

机译：生物转化条件对Amycolatopsis sp。香草醛生产的影响。通过分析竞争性副产物形成的ATCC 39116

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摘要

This study investigated the effects of transformation conditions such as initial pH, the initial concentration of glucose and yeast extract in the medium, and the separate addition of ferulic acid and vanillic acid, on the production of vanillin through an analysis of competing byproduct formation by Amycolatopsis sp. ATCC 39116. The extent and nature of by-product formation and vanillin yield were affected by initial pH and different initial concentrations of glucose and yeast extract in the medium, with a high yield of vanillin and high cell density obtained at pH 8.0, 10 g/l glucose, and 8 g/l yeast extract. High concentrations of ferulic acid were found to negatively affect cell density. Additional supplementation of 100 mg/l vanillic acid, a metabolically linked by-product, was found to result in a high concentration of vanillin and guaiacol, an intermediate of vanillin. Via an analysis of the effect of these transformation conditions on competing by-product formation, high concentrations of ferulic acid were transformed with a molar yield to vanillin of 96.1 and 95.2 %, by Amycolatopsis sp. ATCC 39116 and Streptomyces V1, respectively, together with a minor accumulation of byproducts. These are among the highest performance values reported in the literature to date for Streptomyces in batch cultures.
机译：这项研究通过分析淀粉样芽胞杆菌竞争性副产物的形成，研究了转化条件（例如初始pH，培养基中葡萄糖和酵母提取物的初始浓度以及分别添加阿魏酸和香草酸）对香草醛生产的影响。 sp。 ATCC 39116.初始pH以及培养基中葡萄糖和酵母提取物的初始浓度不同，副产物形成的程度和性质以及香兰素的产量受到影响，在pH 8.0、10 g下，香兰素的产量高且细胞密度高/ l葡萄糖和8 g / l酵母提取物。发现高浓度的阿魏酸会对细胞密度产生负面影响。发现额外补充100 mg / l的代谢相关副产物香草酸会导致高浓度的香草醛和愈创木酚（香草醛的中间体）。通过分析这些转化条件对竞争性副产物形成的影响，由淀粉样芽孢杆菌属菌种将高浓度的阿魏酸转化为香兰素的摩尔产率分别为96.1％和95.2％。分别是ATCC 39116和Streptomyces V1，以及少量副产物。这些是迄今为止在分批培养中链霉菌的文献中报道的最高性能值。

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