...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp. nitrilase
【24h】

Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp. nitrilase

机译：使用表达Alcaligenes sp的戊二醛交联的大肠杆菌细胞有效生产（R）-（-）-扁桃酸。腈水解酶

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recombinant Escherichia coli cells expressing Alcaligenes sp. nitrilase were simply immobilized by direct cross-linking using glutaraldehyde. About 85 % of the total nitrilase activity was recovered under the optimal cross-linking conditions. The thermal stabilities of the cross-linked cells measured at 30, 40 and 50 ℃ were 4.5-, 5.3-, and 5.1-fold those of the free cells, respectively. The concentration of (R)-(-)-mandelic acid reached 280 mM after merely 2 h transformation with the immobilized cells using 300 mM mandelonitrile as substrate, affording an extremely high productivity of 510.7 g L~(-1) d~(-1). In addition, operational stability of the immobilized cells was obviously superior to that of free cells, without significant activity loss after 15 cycles of batch reactions or 8 cycles of repeated fed-batch reactions. Therefore, the easy preparation and robust characteristics of the immobilized biocat-alyst make it a very promising biocatalyst for high-performance and low-cost production of optically pure (R)-(-)-mandelic acid.
机译：表达Alcaligenes sp。的重组大肠杆菌细胞。通过使用戊二醛的直接交联简单地固定腈水解酶。在最佳的交联条件下，回收了总腈水解酶活性的约85％。在30、40和50℃下测得的交联细胞的热稳定性分别为游离细胞的4.5-，5.3-和5.1-倍。 （R）-（-）-扁桃酸的浓度仅用固定化细胞以300 mM扁桃腈为底物转化2 h后就达到280 mM，提供了510.7 g L〜（-1）d〜（-）的极高生产率。 1）。另外，固定化细胞的操作稳定性明显优于游离细胞，在分批反应15个循环或重复补料分批反应8个循环后没有明显的活性损失。因此，固定化生物催化剂的容易制备和稳定的特性使其成为用于高性能和低成本生产光学纯的（R）-（-）-扁桃酸的非常有前途的生物催化剂。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第7期|1241-1248|共8页
  • 作者

    Zhi-Jun Zhang; Jiang Pan; Chun-Xiu Li; Hui-Lei Yu; Gao-Wei Zheng; Xin Ju; Jian-He Xu;

  • 作者单位

    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Immobilized cells; Alcaligenes sp. nitrilase; Glutaraldehyde cross-linking; Enantioselective hydrolysis; (R)-(-)-Mandelic acid;

    机译：固定细胞;产碱菌腈水解酶戊二醛交联;对映选择性水解;（R）-（-）-扁桃酸;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号