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首页> 外文期刊>Bioprocess and Biosystems Engineering >Investigation of continuous-batch mode of two-stage culture of Nannochloropsis sp. for lipid production
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Investigation of continuous-batch mode of two-stage culture of Nannochloropsis sp. for lipid production

机译:拟南芥两阶段培养的连续分批模式研究。用于脂质生产

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摘要

Microalgal lipid induction through nitrogen stress often suffers from a contradiction between biomass productivity and lipid content, i.e., either high biomass productivity with low lipid content or vice versa. A two-stage nitrogen-replete and nitrogen-deplete (NR-ND) culture was suggested to be an option to attain high lipid productivity. In this study, the effects of culture conditions and modes on biomass productivity and lipid productivity of Nannochloropsis sp. in the two stages were comprehensively investigated. The optimal culture conditions for the two stages, aiming to high biomass productivity and lipid productivity respectively, were consistent, i.e., CO_2 content in aeration (1 %), phosphorus concentration in medium (181 umol/L), incident light intensity (150 μE/ (m~2s)), temperature (25 ℃). Different culture modes of the two stages were compared. The overall lipid productivity of the two-stage continuous-batch mode achieved 0.123 g/ (L day), which was 60.3, 48.2, 34.9 and 13.5 % higher than that of single nitrogen-replete batch, single nitrogen- limited batch, continuous nitrogen-replete culture and two-stage batch-batch culture, respectively, and also higher than most reported values. This contribution provides fundamental data for the two-stage NR-ND cultivation process design of Nannochloropsis sp.
机译:通过氮胁迫诱导的微藻类脂质常常遭受生物质生产力和脂质含量之间的矛盾,即,要么高生物质生产力,要么具有低脂质含量,反之亦然。建议使用两阶段的富氮和贫氮(NR-ND)培养来获得高脂质生产率。在这项研究中,培养条件和方式对拟南芥生物量生产力和脂质生产力的影响。在两个阶段进行了全面调查。分别针对高生物量生产率和脂质生产率的两个阶段的最佳培养条件是一致的,即通气中的CO_2含量(1%),培养基中的磷浓度(181 umol / L),入射光强度(150μE) /(m〜2s)),温度(25℃)。比较了两个阶段的不同培养模式。两阶段连续分批模式的总脂质生产率达到0.123 g /(L日),比单氮充足批次,单氮有限批次,连续氮分别高60.3%,48.2%,34.9%和13.5%。完全培养和两阶段分批培养,并且也高于大多数报道的值。这为Nannochloropsis sp。的两阶段NR-ND栽培工艺设计提供了基础数据。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第10期|2073-2082|共10页
  • 作者单位

    National Key Laboratory of Biochemical Engineering,Institute of Process Engineering, Chinese Academy of Sciences,Beijing 100190, China,University of Chinese Academy of Sciences,Beijing 100049, China;

    National Key Laboratory of Biochemical Engineering,Institute of Process Engineering, Chinese Academy of Sciences,Beijing 100190, China;

    National Key Laboratory of Biochemical Engineering,Institute of Process Engineering, Chinese Academy of Sciences,Beijing 100190, China;

    National Key Laboratory of Biochemical Engineering,Institute of Process Engineering, Chinese Academy of Sciences,Beijing 100190, China;

    Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology,Beijing 100029, China;

    National Key Laboratory of Biochemical Engineering,Institute of Process Engineering, Chinese Academy of Sciences,Beijing 100190, China;

    National Key Laboratory of Biochemical Engineering,Institute of Process Engineering, Chinese Academy of Sciences,Beijing 100190, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Lipid productivity; Two-stage cultivation; Nannochloropsis sp.; Continuous culture; Medium recycle;

    机译:脂质生产率;两阶段栽培;Nannochloropsis sp .;持续文化;中等回收;

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