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Lipase production by Aspergillus ibericus using olive mill wastewater

机译：利比亚曲霉利用橄榄磨废水生产脂肪酶

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摘要

Olive mill wastewater (OMW) characteristics make it a suitable resource to be used as a microbial culture media to produce value-added compounds, such as enzymes. In this work, the ability of the novel species Aspergillus ibericus to discolor OMW and produce lipase was studied. An initial screening on plates containing an OMW-based agar medium and an emulsified olive oil/ rhodamine-B agar medium was employed to select the strain A. ibericus MUM 03.49. Then, experiments in conical flasks with liquid OMW-based media showed that the fungus could growth on undiluted OMW, with a chemical oxygen demand (COD) of 97 ± 2 g/L, and to produce up to 2,927 ± 54 U/L of lipase. When pure OMW was used in the media, the maximum COD and color reduction achieved were 45 and 97 %, respectively. When OMW diluted to 10 % was used, A. ibericus was able to reduce phenolic and aromatic compounds by 37 and 39 %, respectively. Additionally, lipase production was found to be promoted by the addition of mineral nutrients. When the fermentations were scaled up to a 2-L bioreactor, A. ibericus produced up to 8,319 ± 33 U/L of lipase, and the maximum COD and color reduction were 57 and 24 %, respectively.
机译：橄榄厂废水（OMW）的特性使其成为用作微生物培养基以生产增值化合物（例如酶）的合适资源。在这项工作中，研究了新物种ibericus ibericus使OMW脱色并产生脂肪酶的能力。在包含基于OMW的琼脂培养基和乳化的橄榄油/若丹明-B琼脂培养基的平板上进行初步筛选，以选择菌株A.ibericus MUM 03.49。然后，在基于OMW液体培养基的锥形烧瓶中进行的实验表明，该真菌可以在未稀释的OMW上生长，化学需氧量（COD）为97±2 g / L，最多可产生2,927±54 U / L。脂肪酶。当在介质中使用纯OMW时，获得的最大COD和减色分别为45％和97％。当使用稀释至10％的OMW时，A。ibericus能够分别还原37％和39％的酚类和芳香族化合物。另外，发现通过添加矿物质营养素可以促进脂肪酶的产生。当发酵规模扩大到2 L生物反应器时，A。ibericus产生的脂肪酶高达8,319±33 U / L，并且最大的COD和颜色减少分别为57％和24％。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2013年第3期|285-291|共7页
  • 作者

    Luis Abrunhosa; Felisbela Oliveira; Danielle Dantas; Cristiana Goncalves; Isabel Belo;

  • 作者单位

    Institute for Biotechnology and Bioengineering (IBB),Centre of Biological Engineering, University of Minho,Campus de Gualtar, 4710-057 Braga, Portugal;

    Institute for Biotechnology and Bioengineering (IBB),Centre of Biological Engineering, University of Minho,Campus de Gualtar, 4710-057 Braga, Portugal;

    Institute for Biotechnology and Bioengineering (IBB),Centre of Biological Engineering, University of Minho,Campus de Gualtar, 4710-057 Braga, Portugal;

    Institute for Biotechnology and Bioengineering (IBB),Centre of Biological Engineering, University of Minho,Campus de Gualtar, 4710-057 Braga, Portugal;

    Institute for Biotechnology and Bioengineering (IBB),Centre of Biological Engineering, University of Minho,Campus de Gualtar, 4710-057 Braga, Portugal;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    olive mill wastewater; aspergillus ibericus; lipase; chemical oxygen demand; submerged fermentation;

    机译：橄榄磨坊废水;伊比利亚曲霉脂肪酶化学需氧量深层发酵;

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