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首页> 外文期刊>Bioprocess and Biosystems Engineering >Extraction and purification of anthraquinones derivatives from Aloe vera L. using alcohol/salt aqueous two-phase system
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Extraction and purification of anthraquinones derivatives from Aloe vera L. using alcohol/salt aqueous two-phase system

机译：乙醇/盐水溶液两相系统提取和纯化芦荟中的蒽醌衍生物

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摘要

An alcohol/salt aqueous two-phase system (ATPS) composed of 1-propanol and (NH_4)_2SO_4 was employed to purify anthraquinones (AQs) extracted from Aloe vera L. The main influencing system parameters such as type of alcohol, type and concentration of salt, temperature and pH were investigated in detail. Under the optimal extraction conditions, AQs can be extracted into alcohol-rich phase with high extraction efficiency, meanwhile majority polysaccharides, proteins, mineral substances and other impurities were extracted into salt-rich phase. Partitioning of AQs is dependent on hydrophobic interaction, hydrogen bond interaction, and salting-out effect in ATPS. Temperature also played a great role in the partitioning. After ATPS extraction, alcohol can be recycled by evaporation; moreover, salt can be recycled by dilution crystallization method. Compared with other liquid-liquid extractions, this alcohol/salt system is much simpler, lower in cost with easier recovery of phase-forming components, which has the potential scale-up in down-processing of active ingredients in plant.
机译：采用由1-丙醇和（NH_4）_2SO_4组成的醇盐水两相系统（ATPS）纯化从芦荟中提取的蒽醌（AQs）。主要影响系统参数，如醇的类型，类型和浓度详细研究了盐，温度和pH值。在最佳提取条件下，可以将AQs提取到富醇相中，提取效率高，同时将多数多糖，蛋白质，矿物质和其他杂质提取到富盐相中。 AQ的分区取决于ATPS中的疏水相互作用，氢键相互作用和盐析作用。温度在分区中也起着重要作用。提取ATPS后，可以通过蒸发将酒精再循环；而且，盐可以通过稀释结晶法再循环。与其他液-液萃取相比，这种醇/盐系统简单得多，成本更低，而且相形成组分的回收更容易，这在植物中有效成分的后处理方面具有潜在的规模。

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