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首页> 外文期刊>Bioprocess and Biosystems Engineering >Batch, fed-batch and repeated fed-batch fermentation processes of the marine thraustochytrid Schizochytrium sp. for producing docosahexaenoic acid
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Batch, fed-batch and repeated fed-batch fermentation processes of the marine thraustochytrid Schizochytrium sp. for producing docosahexaenoic acid

机译：海洋破囊壶菌Schizochytrium sp。的分批，补料和分批补料发酵过程。用于生产二十二碳六烯酸

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摘要

Different fermentation processes, including batch, fed-batch and repeated fed-batch processes by Schizochytrium sp., were studied and compared for the effective DHA-rich microbial lipids production. The comparison between different fermentation processes showed that fed-batch process was a more efficient cultivation strategy than the batch process. Among the four different feeding strategies, the glucose concentration feed-back feeding strategy had achieved the highest fermentation results of final cell dry weight, total lipids content, DHA content and DHA productivity of 72.37, 48.86, 18.38 g l~(-1) and 138.8 mg l~(-1) h~(-1) respectively. The repeated fed-batch process had the advantages of reducing the time and cost for seed culture and inoculation between each fermentation cycles. The results of fermentation characteristics and lipid characterization of the repeated fed-batch process indicated that this repeated fed-batch process had promising industrialization prospect for the production of DHA-rich microbial lipids.
机译：研究了不同的发酵过程，包括Schizochytrium sp。的分批，补料分批和重复补料分批工艺，并比较了富含DHA的有效微生物脂质的生产。不同发酵工艺之间的比较表明，分批补料工艺比分批工艺更有效。在四种不同的饲喂策略中，葡萄糖浓度反馈饲喂策略在最终细胞干重，总脂质含量，DHA含量和DHA生产率方面的发酵效果最高，分别为72.37、48.86、18.38 gl〜（-1）和138.8。毫克l〜（-1）h〜（-1）。重复的分批补料工艺具有减少种子培养和每个发酵周期之间接种的时间和成本的优点。重复分批补料工艺的发酵特性和脂质表征结果表明，该重复分批补料工艺具有生产富含DHA的微生物脂质的工业化前景。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2013年第12期|1905-1912|共8页
  • 作者

    Liang Qu; Lu-Jing Ren; Guan-Nan Sun; Xiao-Jun Ji; Zhi-Kui Me; He Huang;

  • 作者单位

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China;

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  • 正文语种 eng
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  • 关键词

    Schizochytrium sp.; Docosahexaenoic acid; Feeding strategy; Repeated fed-batch;

    机译：裂殖壶菌属;二十二碳六烯酸;喂养策略;重复补料;

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