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首页> 外文期刊>Bioprocess and Biosystems Engineering >Optimization of the mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-)
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Optimization of the mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-)

机译：Blakeslea trispora NRRL 2895（+）和NRRL 2896（-）对番茄红素生产的配合发酵工艺的优化

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摘要

The mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-) was systematically optimized in shake flasks. The ratio of the (+) to (-) strains, the lycopene cyclase inhibitors piperidine and creatinine, the trisporic acid structural analog abscisic acid, the 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) precursor leu-cine, and the mevalonate kinase enhancer penicillin were all identified as key factors affecting lycopene biosynthesis. With an optimal ratio of 5:1 for the (+) to (-) strains and the addition of 6 g/L creatinine on day 3, the highest lycopene production was 98.1 ± 15.5 mg/L. Based on the above result, the addition of 0.1 g/L penicillin on day 4, 150 μrnol/L abscisic acid on day 3 or 0.5 g/L leucine on day 4 enhanced lycopene production to 119.7 ± 17.2, 120.6 ± 12.3 and 135.2 ± 7.0 mg/L, respectively. Finally, an integrated strategy by combining the above key factors was developed, and the highest lycopene production of 156.2 ±15.4 mg/L was obtained, which was enhanced by 134.9% comparing with its production of 66.5 ± 3.6 mg/L before the optimization process of this work. The results obtained in this study may be useful for large-scale industrial lycopene production.
机译：在摇瓶中系统地优化了Blakeslea trispora NRRL 2895（+）和NRRL 2896（-）生产番茄红素的配合发酵过程。 （+）与（-）菌株，番茄红素环化酶抑制剂哌啶和肌酸酐，三孢子酸结构类似物脱落酸，3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）前体亮氨酸的比例，甲羟戊酸激酶增强剂青霉素都被认为是影响番茄红素生物合成的关键因素。对于（+）与（-）菌株，最佳比例为5：1，并在第3天添加6 g / L肌酐，番茄红素的最高产量为98.1±15.5 mg / L。根据上述结果，第4天添加0.1 g / L青霉素，第3天添加150μnol/ L脱落酸或第4天添加0.5 g / L亮氨酸可将番茄红素的产量提高至119.7±17.2、120.6±12.3和135.2±分别为7.0 mg / L。最后，结合上述关键因素，制定了综合策略，番茄红素的最高产量为156.2±15.4 mg / L，比优化前的66.5±3.6 mg / L产量提高了134.9％。这项工作。这项研究中获得的结果可能对大规模工业番茄红素生产有用。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第4期|p.553-564|共12页
  • 作者

    Jin-Feng Wang; Xiu-Ji Liu; Rui-Sang Liu; Hong-Mei Li; Ya-Jie Tang;

  • 作者单位

    Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology,Wuhan 430068, China;

    Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology,Wuhan 430068, China;

    Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology,Wuhan 430068, China;

    Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology,Wuhan 430068, China;

    Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology,Wuhan 430068, China,Lab of Biorefinery Engineering, Shanghai Advanced Research Institute, Chinese Academy of Sciences,Shanghai 201203, China,National Key Laboratory of Biochemical Engineering,Institute of Process Engineering, Chinese Academy of Sciences,Beijing 100080, China,State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,130 Meilong Road, Shanghai 200237, China;

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  • 正文语种 eng
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  • 关键词

    lycopene; the mated fermentation of blakeslea trispora; process optimization;

    机译：番茄红素;Blakeslea trispora的配合发酵;工艺优化;

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