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首页> 外文期刊>Bioprocess and Biosystems Engineering >Small microcapsules of crystal proteins and spores of Bacillus thuringiensis by an emulsification/internal gelation method
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Small microcapsules of crystal proteins and spores of Bacillus thuringiensis by an emulsification/internal gelation method

机译：苏云金芽孢杆菌晶体蛋白和孢子的微囊乳化/内凝胶法

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摘要

This paper describes a microencapsulation process of a spore crystal aggregate produced by Bacillus thuringiensis var. kurstaki HD-1. The methodology is based on the emulsification/internal gelation method, and was implemented to produce microcapsules of small diameter (<10 μm) with the capacity to protect the spore crystal aggregate from extreme ultraviolet radiation. The diameter of microcapsules was in the range of 3.1 ± 0.2-6.8 ± 0.4 μm, which is considered adequate for biological control purposes. The protective effect of the alginate coat was verified by the remaining 60 ± 2% and 40 ± 1% of spore viability and protein activity, respectively, after UV-B radiation of 236 J, and with bioassays with Spodoptera frugiperda. It is expected that the protective effect of the alginate coat will improve the effectiveness of the Bt-HD1 formulated as small diameter microcapsules, and their yield, once they are released into the environment, will also be improved.
机译：本文描述了苏云金芽胞杆菌产生的孢子晶体聚集体的微囊化过程。库尔斯塔基HD-1。该方法基于乳化/内部凝胶化方法，并用于生产小直径（<10μm）的微囊，能够保护孢子晶体聚集体免受极端紫外线辐射。微胶囊的直径在3.1±0.2-6.8±0.4μm的范围内，被认为足以用于生物学控制。在236 J的UV-B辐射后，以及用节食夜蛾（Spodoptera frugiperda）进行生物测定，分别通过剩余的60±2％和40±1％的孢子生存力和蛋白质活性验证了藻酸盐涂层的保护作用。预期藻酸盐涂层的保护作用将改善配制成小直径微胶囊的Bt-HD1的有效性，并且一旦将它们释放到环境中，它们的产率也将得到改善。

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