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首页> 外文期刊>Bioprocess and Biosystems Engineering >A novel biotransformation process of 4'-demethylepipodo-phyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization
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A novel biotransformation process of 4'-demethylepipodo-phyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization

机译：新型芽孢杆菌CICC 20463将4'-脱甲基表鬼臼毒素转化为4'-脱甲基表鬼臼酸的新生物转化方法，第二部分：工艺优化

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摘要

This work optimized the novel biotransformation process of 4'-demethylepipodophyllotoxin (DMEP) into 4'-demethylepipodophyllic acid (DMEPA) by Bacillus fusiformis CICC 20463. Firstly, the biotransformation process was significantly affected by medium composition. 5 g/L of yeast extract and 10 g/L of peptone were optimal for DMEPA production (i.e., 2.81 ± 0.21 mg/L), while not beneficial for the cell growth of B. fusiformis. This indicated that the biosynthesis of DMEPA was not corresponded well to the cell growth of B. fusiformis. 40 g/L of sucrose was optimal for DMEPA production (i.e., 2.94 ±0.17 mg/L), and 3 g/L of NaCl was the best for DMEPA production (i.e., 4.10 ± 0.18 mg/L). Secondly, the production of DMEPA was significantly enhanced by the control of substrate concentration and culture pH. 100 mg/L of substrate was optimal for DMEPA production (i.e., 6.47 ± 0.35 mg/L), and DMEPA concentration was enhanced to 38.78 mg/L by controlling culture pH at 9.0 in the stirred-tank bioreactors. The fundamental informationrnobtained in this study provides a simple and efficient way to produce DMEPA by biotransformation.
机译：这项工作优化了新型芽孢杆菌CICC 20463将4'-脱甲基表鬼臼毒素（DMEP）转化为4'-脱甲基表鬼臼酸（DMEPA）的生物转化过程。首先，生物转化过程受到培养基组成的显着影响。 5克/升的酵母提取物和10克/升的蛋白for最适于DMEPA的生产（即2.81±0.21毫克/升），而对梭形芽孢杆菌的细胞生长无益。这表明DMEPA的生物合成与梭形芽孢杆菌的细胞生长不完全对应。 40克/升的蔗糖最适合用于DMEPA的生产（即2.94±0.17毫克/升），而3克/升的氯化钠最适合用于DMEPA的生产（即4.10±0.18毫克/升）。其次，通过控制底物浓度和培养液pH可以显着提高DMEPA的产量。 100 mg / L的底物最适合用于DMEPA的生产（即6.47±0.35 mg / L），通过将搅拌罐生物反应器中的培养物pH控制在9.0，可以将DMEPA的浓度提高到38.78 mg / L。本研究中获得的基本信息为通过生物转化生产DMEPA提供了一种简单有效的方法。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2010年第2期|237-246|共10页
  • 作者

    Ya-Jie Tang; Xiao-Ling Xu; Jian-Jiang Zhong;

  • 作者单位

    Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, 430068 Wuhan, China National Key Laboratory of Biochemical Engineering,Institute of Process Engineering, Chinese Academy of Sciences,100080 Beijing, China;

    Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, 430068 Wuhan, China;

    Key Laboratory of Microbial Metabolism (Ministry of Education). College of Life Science & Biotechnology,Shanghai Jiao Tong University, 200240 Shanghai, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    biotransformation process optimization; 4'-Demethylepipodophyllotoxin; bacillus fusiformis; 4'-demethylepipodophyllic acid;

    机译：生物转化过程优化;4'-脱甲基表鬼臼毒素;梭状芽孢杆菌;4'-去甲基表鬼臼酸;

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