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首页> 外文期刊>Bioprocess and Biosystems Engineering >Impact of oxygen level in gaseous phase on gene transcription and ganoderic acid biosynthesis in liquid static cultures of Ganoderma lucidum
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Impact of oxygen level in gaseous phase on gene transcription and ganoderic acid biosynthesis in liquid static cultures of Ganoderma lucidum

机译：灵芝液体静态培养物中气相氧水平对基因转录和灵芝酸生物合成的影响

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摘要

Liquid static cultivation of Ganoderma lucidum was previously found to be very efficient for improving the production of its valuable antitumor compound ganoderic acid (GA) (Fang and Zhong in Biotechnol Prog 18:51-54, 2002). In this work, effects of oxygen concentration within the range of 21-100% (v/v) in the gaseous phase on the mycelia growth, GA production, and gene transcription of key enzymes for GA biosynthesis in liquid static cultures of G. lucidum were investigated. A high cell density of 29.8 ± 1.7 g/l DW and total GA production of 1427.2 ± 74.2 mg/l were obtained under an optimal gaseous O_2 level of 80%. The expression of 3-hydroxy-3-methyl-glutaryl-CoA reductase, squalene synthase and lanosterol synthase genes of GA biosynthetic pathway as detected by quantitative real-time PCR was also affectedrnby the gaseous oxygen concentration in the liquid static culture. H_2O_2 was generated as reactive oxygen species in response to high oxygen concentrations in the gas phase, and it seemed to be involved in the regulation of GA biosynthesis. The information obtained in this study provided an insight into the role of gaseous O_2 in the GA production and it will be helpful for further enhancing its productivity.
机译：先前发现灵芝的液体静态培养对于提高其有价值的抗肿瘤化合物灵芝酸（GA）的生产非常有效（Fang and Zhong in Biotechnol Prog 18：51-54，2002）。在这项工作中，气相中氧气浓度在21-100％（v / v）范围内对灵芝液体静态培养物中菌丝体生长，GA产生以及GA生物合成关键酶基因转录的影响。被调查了。在80％的最佳气态O_2水平下，获得了29.8±1.7 g / l DW的高细胞密度和1427.2±74.2 mg / l的总GA产量。定量实时荧光定量PCR检测GA生物合成途径中3-羟基-3-甲基-戊二酰-CoA还原酶，角鲨烯合酶和羊毛甾醇合酶基因的表达也受到液体静态培养物中气态氧浓度的影响。 H_2O_2响应于气相中的高氧浓度而作为活性氧生成，并且似乎参与了GA生物合成的调控。这项研究中获得的信息提供了对气态O_2在GA生产中的作用的深入了解，这将有助于进一步提高其生产率。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2010年第6期|P.683-690|共8页
  • 作者

    Wen-Xian Zhang; Ya-Jie Tang; Jian-Jiang Zhong;

  • 作者单位

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, China Engineering Research Center of Industrial Microbiology, Ministry of Education, College of Life Sciences, Fujian Normal University, 350108 Fuzhou, China;

    rnKey Laboratory of Fermentation Engineering (Ministry of Education) and Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, 430068 Wuhan, China;

    rnState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, China Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, 200240 Shanghai, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    medicinal mushroom fermentation; liquid static culture; ganoderma lucidum; ganoderic acid production; gene expression;

    机译：药用蘑菇发酵液体静态培养;灵芝灵芝酸的生产;基因表达;

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