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首页> 外文期刊>Bioprocess and Biosystems Engineering >Influence of L-isoleucine and pantothenate auxotrophy for L-valine formation in Corynebacterium glutamicum revisited by metabolome analyses
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Influence of L-isoleucine and pantothenate auxotrophy for L-valine formation in Corynebacterium glutamicum revisited by metabolome analyses

机译：代谢组学分析重新探讨L-异亮氨酸和泛酸营养缺陷型对谷氨酸棒状杆菌L-缬氨酸形成的影响

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摘要

The effect of different amounts of supplemented L-isoleucine and pantothenate has been analysed with the auxotrophic strain Corynebacterium glutamicum △ilvA △panB, showing that the final biomass concentration of this preliminary L-valine production strain can be controlled by the amount of added L-isoleucine. One gramme cell dry weight is formed from 48 μmol L-isoleucine. Different amounts of available pantothenate affect the intracellular pyruvate concentration. By limiting pantothenate supplementation from 0.8 to 0.1 μM, a 35-fold increase of cytoplasmic pyruvate up to 14.2 mM can be observed, resulting in the increased formation of L-valine, L-alanine and organic acids in the presence of low pantothenate concentrations. These findings can be used to redirect the carbon flux from glycolysis via pyruvate to the TCA cycle towards the desired product L-valine.
机译：用营养缺陷型菌株谷氨酸棒杆菌△ilvA△panB分析了不同量的补充L-异亮氨酸和泛酸的作用，表明该初步的L-缬氨酸生产菌株的最终生物量浓度可以通过添加L-的量来控制。异亮氨酸。一克细胞干重由48μmolL-异亮氨酸形成。不同数量的可用泛酸影响细胞内丙酮酸的浓度。通过将泛酸的添加量限制为0.8至0.1μM，可以观察到胞质丙酮酸的35倍增加，至14.2 mM，导致在低泛酸浓度下L-缬氨酸，L-丙氨酸和有机酸的形成增加。这些发现可用于将碳通量从糖酵解通过丙酮酸重新导向到TCA循环，再流向所需的产物L-缬氨酸。

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