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首页> 外文期刊>Bioprocess and Biosystems Engineering >Study of the inhibitory effect of the product dihydroxyacetone on Gluconobacter oxydans in a semi-continuous two-stage repeated-fed-batch process
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Study of the inhibitory effect of the product dihydroxyacetone on Gluconobacter oxydans in a semi-continuous two-stage repeated-fed-batch process

机译：半连续两阶段重复进料分批过程中产物二羟基丙酮对氧化葡糖杆菌的抑制作用的研究

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摘要

The influence of the product inhibition by dihydroxyacetone (DHA) on Gluconobacter oxydans for a novel semi-continuous two-stage repeated-fed-batch process was examined quantitatively. It was shown that the culture was able to grow up to a DHA concentration of 80 kg m~(-3) without any influence of product inhibition. The regeneration capability of the reversibly product inhibited culture from a laboratory-scale bio-reactor system was observed up to a DHA concentration of about 160 kg m~(-3). At higher DHA concentrations, the culture was irreversibly product inhibited. However, due to the robust membrane-bound glycerol dehydro-genase of G. oxydans, product formation was still active for a prolonged period of time. The reachable maximum final DHA concentration was as high as 220 kg m~(-3). The lag phases for growth increased exponentially with increasing DHA threshold values of the first reactor stage. These results correlated well with fluorescence in situ hybridization (FISH) measurements confirming that the number of active cells decreased exponentially with increasing DHA concentrations.
机译：定量检查了新颖的半连续两阶段重复进料分批工艺中，二羟基丙酮（DHA）抑制产物对氧化葡糖杆菌的影响。结果表明，该培养物能够生长至DHA浓度达80 kg m〜（-3），而不受产物抑制的影响。观察到在实验室规模的生物反应器系统中可逆产物抑制培养物的再生能力，直至DHA浓度达到约160 kg m〜（-3）。在较高的DHA浓度下，不可逆地抑制了产物的培养。但是，由于氧化羟色胺的牢固的膜结合甘油脱氢酶，产物形成在很长一段时间内仍然是活跃的。最终最大DHA浓度可达220 kg m〜（-3）。随着第一反应器阶段DHA阈值的增加，生长的滞后阶段呈指数增长。这些结果与荧光原位杂交（FISH）测量结果非常相关，这证实了活性细胞的数量随DHA浓度的增加呈指数下降。

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