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首页> 外文期刊>Bioprocess and Biosystems Engineering >Synthesis of a green polyurethane foam from a biopolyol obtained by enzymatic glycerolysis and its use for immobilization of lipase NS-40116
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Synthesis of a green polyurethane foam from a biopolyol obtained by enzymatic glycerolysis and its use for immobilization of lipase NS-40116

机译：由酶促糖酵解获得的生物多元醇合成绿色聚氨酯泡沫及其在脂肪酶NS-40116固定化中的应用

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摘要

The use of green sources for materials synthesis has gained popularity in recent years. This work investigated the immobilization of lipase NS-40116 (Thermomyces lanuginosus lipase) in polyurethane foam (PUF) using a biopolyol obtained through the enzymatic glycerolysis between castor oil and glycerol, catalyzed by the commercial lipase Novozym 435 for the PUF formation. The reaction was performed to obtain biopolyol resulting in the conversion of 64% in mono- and diacylglycerol, promoting the efficient use of the reaction product as biopolyol to obtain polyurethane foam. The enzymatic derivative with immobilized lipase NS-40116 presented apparent density of 0.19 +/- 0.03g/cm(3) and an immobilization yield was 94 +/- 4%. Free and immobilized lipase NS-40116 were characterized in different solvents (methanol, ethanol, and propanol), temperatures (20, 40, 60 and 80 degrees C), pH (3, 5, 7, 9 and 11) and presence of ions Na+, Mg++, and Ca++. The support provided higher stability to the enzyme, mainly when subjected to acid pH (free lipase lost 80% of relative activity after 360h of contact, when the enzymatic derivative lost around 22%) and high-temperature free lipase lost 50% of relative activity, while the immobilized remained 95%. The enzymatic derivative was also used for esterification reactions and conversions around 66% in fatty acid methyl esters, using abdominal chicken fat as feedstock, were obtained in the first use, maintaining this high conversion until the fourth reuse, proving that the support obtained using environmentally friendly techniques is applicable.
机译：近年来，将绿色来源用于材料合成已变得普及。这项工作研究了使用生物多元醇将脂肪酶NS-40116（羊毛嗜热菌脂肪酶）固定在聚氨酯泡沫塑料（PUF）中的方法，该生物多元醇是通过蓖麻油和甘油之间的酶促甘油分解酶解获得的，该生物酚由商用脂肪酶Novozym 435催化形成PUF。进行反应以获得生物多元醇，导致单酰基和二酰基甘油的转化率为64％，从而促进了反应产物作为生物多元醇的有效利用以获得聚氨酯泡沫。具有固定化脂肪酶NS-40116的酶衍生物的表观密度为0.19 +/- 0.03g / cm（3），固定化产率为94 +/- 4％。游离和固定化脂肪酶NS-40116的特征在于不同的溶剂（甲醇，乙醇和丙醇），温度（20、40、60和80摄氏度），pH（3、5、7、9和11）和离子的存在Na +，Mg ++和Ca ++。支持物为酶提供了更高的稳定性，主要是在酸性pH下（接触360h后游离脂肪酶丧失了80％的相对活性，当酶衍生物损失了约22％时），高温游离脂肪酶丧失了50％的相对活性。 ，而固定人数仍为95％。该酶衍生物也用于酯化反应，并在第一次使用时以腹部鸡脂肪为原料获得了约66％的脂肪酸​​甲酯转化率，一直保持这种高转化率直到第四次重复使用，证明了使用环境获得的载体友好的技术是适用的。

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